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Updated: Feb 23, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

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Published on: December 10, 2016

CD2068 potentially mediates multidrug efflux in Clostridium difficile.

Chawalit Ngernsombat¹, Suthasinee Sreesai², Phurt Harnvoravongchai¹

¹Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.

Scientific Reports

|September 1, 2017

Summary

A novel ABC transporter, CD2068, contributes to multidrug resistance in Clostridium difficile. This discovery offers new insights into combating antibiotic-resistant infections and developing effective treatments for C. difficile infections.

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A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

Area of Science:

Microbiology
Molecular Biology
Drug Resistance

Background:

Clostridium difficile causes antibiotic-associated diarrhea, complicated by multidrug-resistant strains.
ATP-binding cassette (ABC) transporters are implicated in microbial multidrug resistance.

Purpose of the Study:

To investigate the role of the ABC transporter CD2068 in Clostridium difficile multidrug resistance.

Main Methods:

Gene expression analysis of cd2068 in C. difficile exposed to antibiotics.
Heterologous expression of CD2068 in Escherichia coli to assess substrate efflux and drug resistance.
Assay of CD2068-associated ATPase activity.
Insertional inactivation and genetic complementation of the cd2068 gene in C. difficile.

Main Results:

cd2068 gene expression was upregulated by antibiotics.
CD2068 mediated efflux of fluorescent substrates and conferred multidrug resistance in E. coli.
Antibiotics stimulated CD2068-associated ATPase activity.
Disruption of cd2068 increased susceptibility to antibiotics in C. difficile.

Conclusions:

The ABC transporter CD2068 plays a significant role in the multidrug resistance mechanism of Clostridium difficile.
CD2068 is a potential target for developing strategies to overcome antibiotic resistance in C. difficile infections.