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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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qF-SSOP: real-time optical property corrected fluorescence imaging.

Pablo A Valdes1,2, Joseph P Angelo3,4,2, Hak Soo Choi5

  • 1Department of Neurosurgery, Harvard Medical School, Brigham and Women's/Boston Children's Hospitals, Building for Transformative Medicine, 60 Fenwood Road, Boston, MA 02115, USA.

Biomedical Optics Express
|September 1, 2017
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Summary

This study introduces a new fluorescence imaging method that corrects for tissue optical properties in real-time. This quantitative fluorescence imaging technique improves accuracy for surgical guidance in oncology and vascular procedures.

Keywords:
(110.2960) Image analysis(170.3880) Medical and biological imaging

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Area of Science:

  • Medical imaging
  • Optical engineering
  • Surgical technology

Background:

  • Fluorescence imaging aids surgical guidance in oncology and vascular procedures.
  • Current systems struggle with optical property distortions, leading to inaccurate fluorophore concentration estimates.
  • Accurate molecular guidance intraoperatively is crucial for improving surgical outcomes.

Purpose of the Study:

  • To develop a novel fluorescence imaging technique for real-time optical property correction.
  • To provide quantitative fluorescence imaging with high accuracy during surgical procedures.
  • To enhance intraoperative molecular guidance for surgeons.

Main Methods:

  • Simultaneous imaging of tissue optical properties using Single Snapshot of Optical Properties (SSOP) and fluorescence detection.
  • Real-time estimation of optical properties across the full field of view.
  • Correction of emitted fluorescence using a quantitative model based on estimated optical properties.

Main Results:

  • Achieved real-time, optical property-corrected fluorescence imaging at video rates.
  • Generated quantitative fluorescence-Single Snapshot of Optical Properties (qF-SSOP) images with less than 5% error.
  • Demonstrated a rigorous, fast, and quantitative imaging technique.

Conclusions:

  • The developed qF-SSOP technique offers accurate, real-time fluorescence imaging by correcting for optical property distortions.
  • This method can be readily integrated into surgical workflows.
  • The technique has the potential to significantly improve intraoperative molecular guidance and surgical precision.