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Updated: Feb 23, 2026
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
Published on: May 1, 2019
Location of monoclonal peak as a tool in checking specimen integrity
Shikhar G Vyas1, Gurmukh Singh2
1Resident in Pathology, Medical College of Georgia at Augusta University, 1120, 15th Street BI 2008A, Augusta, GA 30912, USA.
Objectives:
Serum protein electrophoresis is a commonly used test in the diagnosis and follow-up of patients with monoclonal gammopathies. The practice of documenting the location of the peak may serve as delta check flags in SPEP samples.
Methods:
We report on the inconsistent finding on two tests performed about three months apart. The inconsistency was discovered due to recognition of the change in the location of the monoclonal immunoglobulin on protein electrophoresis.
Results:
Repeat testing with a third specimen revealed that the first test was run on a wrong specimen.
Conclusion:
Recording the location of the monoclonal spike is recommended to serve as an additional "delta" check.
