Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

759
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
759

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reduced Soluble Ubiquilin2 in Amyotrophic Lateral Sclerosis Carrying Ubiquilin2 (P494L) Mutation: Clinicopathological and Biochemical Evidence From an Autopsy Case.

Neuropathology and applied neurobiology·2026
Same author

A translocation-mediated duplication of the distal region of chromosome 5A reduced the culm length in a gamma-irradiated wheat mutant.

Molecular breeding : new strategies in plant improvement·2026
Same author

A wheat MYB transcription factor activates defense by repressing TaSIZ1 in response to branched-chain amino acid accumulation.

The Plant cell·2026
Same author

Virulence gains in the Puccinia striiformis f. sp. tritici PstS10 lineage correlate with expression polymorphism in a candidate Avr effector.

Communications biology·2026
Same author

Retrospective Study on the Effects of Intensive Speech Therapy Combined with Two Courses of High-Frequency rTMS on Language Function in Patients with Aphasia.

NeuroRehabilitation·2026
Same author

Mechanisms of aroma compound formation in traditional Japanese sake brewing by natural lactic acid fermentation: Insights from solvent-assisted stir bar sorptive extraction with GC-MS and bacterial flora analysis.

Food chemistry·2025

Related Experiment Video

Updated: Feb 23, 2026

Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone
07:36

Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone

Published on: March 17, 2023

2.3K

Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance.

Vanessa Bueno-Sancho1,2, Daniel C E Bunting1,2, Luis J Yanes1

  • 1Earlham Institute, Norwich Research Park, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)
|September 1, 2017
PubMed
Summary

Field Pathogenomics uses RNA-seq on plant samples for pathogen surveillance. This technique reveals pathogen relationships and population structure directly in the field, improving disease diagnostics.

Keywords:
Emerging pathogensFilamentous plant pathogensPathogen surveillancePhylogenomicsPopulation genetics

More Related Videos

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
14:15

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

17.6K
Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
06:12

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

2.5K

Related Experiment Videos

Last Updated: Feb 23, 2026

Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone
07:36

Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone

Published on: March 17, 2023

2.3K
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
14:15

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

17.6K
Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
06:12

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

2.5K

Area of Science:

  • Plant pathology
  • Genomics
  • Bioinformatics

Background:

  • Traditional plant pathogen diagnostics rely on phenotypic or PCR-based methods, which are limited to known agents and can introduce bias.
  • Next-generation sequencing offers advanced tools for high-resolution genotype data in pathogen surveillance.
  • Novel approaches are needed to characterize pathogen populations directly in their natural environment.

Purpose of the Study:

  • To introduce and detail the bioinformatics analyses within the "Field Pathogenomics" technique.
  • To demonstrate the utility of RNA-seq data from field-collected plant samples for pathogen surveillance.
  • To characterize pathogen populations and their host interactions in situ.

Main Methods:

  • RNA-sequencing (RNA-seq) was performed on pathogen-infected plant leaf samples collected directly from the field.
  • Bioinformatics analyses included phylogenetic analysis to determine isolate relationships.
  • Population structure analysis was conducted to understand genetic substructure within pathogen populations.

Main Results:

  • The Field Pathogenomics technique enables direct characterization of pathogen populations in their natural environment.
  • Phylogenetic analysis provided insights into the evolutionary relationships among collected pathogen isolates.
  • Population structure analysis revealed genetic substructure, offering a high-resolution view of pathogen dynamics.

Conclusions:

  • Field Pathogenomics offers a powerful, high-resolution approach for real-time pathogen surveillance and population dynamics studies.
  • This technique enhances our understanding of pathogen biology, population structure, and pathogenesis directly in the field.
  • It represents a significant advancement over traditional methods for diagnosing and monitoring plant pathogens.