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Updated: Feb 23, 2026
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
Published on: August 23, 2013
BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance
1Department of Plant Sciences and Plant Pathology, Montana State University, 307 Plant BioScience Bldg, P.O. Box 173150, Bozeman, MT, 59717, USA. lhuang@montana.edu.
Abstract:
Virus-induced gene silencing (VIGS) is a widely used reverse genetics tool to knock down genes in plants transiently without transformation. The assay has been successfully used to downregulate the transcript abundance of a target gene at almost any plant developmental stages in any tissues. Here, we describe the VIGS assay using a barley stripe mosaic virus (BSMV) for functional genomics analysis in wheat with the focus on genes involved in rust resistance.
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