Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene that Encodes a Hexose...

Recently, the Lr67 resistance gene was identified as a hexose transporter variant which confers adult plant rust and mildew resistance in wheat. Methodologies used to characterize the protein encoded by Lr67 may be of use to non-transporter experts conducting similar experiments with other hexose transporters. Hence, in this chapter, we detail a protocol for the functional characterization of hexose transporter proteins in the Saccharomyces cerevisiae expression system. We also provide guidance on the use of metabolic inhibitors and competing sugars to probe transporter structural features, energization, and specificity.