Chitinophaga sedimenti sp. nov., isolated from sediment

A bacterial strain designated TFL-3T was isolated from sediment of the Yangtze River in Nanjing, Jiangsu province, China. Strain TFL-3T was Gram-staining-negative, non-spore-forming, rod-shaped and non-motile. Growth occurred at 10-40 °C (optimum 30 °C). Strain TFL-3T grew at pH 6.0-9.0 (optimum pH 7.0) and NaCl concentrations of 0-2.5 % (optimum 0.5 %). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain TFL-3T belonged to the genus Chitinophaga and showed the highest sequence similarity to Chitinophaga barathri YLT18T (94.9 %). The DNA G+C content of strain TFL-3T was determined as 50.5±1.0 mol%. The major fatty acids (>10 %) were iso-C15 : 0, C16 : 1ω5c and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The predominant respiratory ubiquinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids and seven unidentified lipids. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain TFL-3T represents a novel species in the genus Chitinophaga, for which the name Chitinophaga sedimenti sp. nov. is proposed. The type strain is TFL-3T (=ACCC 19966T=KCTC 52590T).