Single-Scan ¹³ C Diffusion-Ordered NMR Spectroscopy of DNP-Hyperpolarised Substrates

Area of Science:

• Nuclear Magnetic Resonance (NMR) Spectroscopy
• Analytical Chemistry
• Physical Chemistry

Background:

• Diffusion-ordered NMR spectroscopy (DOSY) is valuable for analyzing molecular mixtures.
• The sensitivity limitations of conventional NMR restrict the application of DOSY.
• Hyperpolarisation techniques offer significant signal enhancement for NMR.

Purpose of the Study:

• To demonstrate spectrally resolved 13C DOSY using hyperpolarised substrates.
• To overcome the sensitivity limitations of conventional DOSY.
• To enable rapid acquisition of multiple DOSY spectra within a single hyperpolarisation experiment.

Main Methods:

• Utilisation of dissolution dynamic nuclear polarisation (D-DNP) for substrate hyperpolarisation.
• Acquisition of spectrally resolved 13C DOSY data in a single scan.
• Employment and numerical simulation of a convection-compensation pulse scheme.

Main Results:

• Achieved signal enhancements of several orders of magnitude via D-DNP.
• Successfully collected spectrally resolved 13C DOSY data in a single scan.
• Demonstrated the capability to acquire multiple consecutive DOSY spectra from a single D-DNP experiment.

Conclusions:

• D-DNP significantly enhances NMR sensitivity, enabling rapid DOSY measurements.
• The proposed method overcomes traditional sensitivity limitations of DOSY.
• This technique expands the applicability of DOSY for analysing complex molecular mixtures efficiently.