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Related Concept Videos

SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

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Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
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Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

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Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
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Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

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The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
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Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

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The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
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Nuclear Protein Sorting01:34

Nuclear Protein Sorting

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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Related Experiment Video

Updated: Feb 23, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
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Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

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SNARE proteins in membrane trafficking.

Tuanlao Wang1, Liangcheng Li1, Wanjin Hong1,2

  • 1School of Pharmaceutical Sciences, State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen, China.

Traffic (Copenhagen, Denmark)
|September 1, 2017
PubMed
Summary
This summary is machine-generated.

Soluble NSF Attachment Protein Receptors (SNAREs) drive membrane fusion. Recent biophysical and single-molecule studies reveal intricate fusion mechanisms, with significant contributions from Chinese researchers advancing our understanding of membrane trafficking.

Keywords:
SNAREmembrane fusionmembrane traffickingtethering factor

More Related Videos

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

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Related Experiment Videos

Last Updated: Feb 23, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

14.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biophysics

Background:

  • Soluble NSF Attachment Protein Receptors (SNAREs) are essential protein complexes that mediate intracellular membrane fusion events.
  • Membrane fusion is a fundamental biological process crucial for various cellular functions, including neurotransmitter release and organelle trafficking.

Purpose of the Study:

  • To provide an updated review on the recent advancements in understanding SNAREs and their role in regulating membrane fusion.
  • To highlight the application of advanced biophysical and single-molecule analysis techniques in dissecting fusion processes.
  • To summarize key research contributions from Chinese laboratories in the field of SNARE-mediated membrane trafficking.

Main Methods:

  • Review of recent scientific literature on SNAREs and membrane fusion.
  • Analysis of studies employing advanced biophysical methods (e.g., single-molecule FRET, force spectroscopy).
  • In vitro single-molecule assays to dissect the dynamics of SNARE complex assembly and function.

Main Results:

  • Detailed insights into the step-by-step mechanisms of SNARE-mediated membrane fusion have been elucidated.
  • Biophysical and single-molecule approaches have provided unprecedented resolution of fusion intermediates and dynamics.
  • Significant progress has been made by Chinese scientists, contributing novel findings to the field of membrane trafficking.

Conclusions:

  • SNAREs represent a conserved and sophisticated machinery for membrane fusion.
  • Advanced biophysical techniques continue to refine our understanding of the molecular details of SNARE function.
  • International research, particularly from China, plays a vital role in advancing the study of SNARE-mediated membrane trafficking.