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Engineering Robustness of Microbial Cell Factories.

Zhiwei Gong^1,2, Jens Nielsen³, Yongjin J Zhou¹

¹Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, P.R. China.

Biotechnology Journal

|September 1, 2017

Summary

Metabolic engineering aims to create robust microbial cell factories for sustainable production. This review covers strategies like pathway balancing and adaptive evolution to overcome industrial limitations and enhance yields.

Keywords:

adaptive laboratory evolution dynamic metabolic engineering microbial cell factories robustness engineering systems and synthetic biology

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Area of Science:

Metabolic Engineering
Synthetic Biology
Microbial Biotechnology

Background:

Metabolic engineering and synthetic biology show promise for microbial cell factories converting renewable resources.
Low production rates and concentrations hinder industrial applications despite optimized biosynthetic pathways.
Host cell survival is compromised by harsh conditions, inhibitors, and toxic compounds, necessitating robust engineered microbes.

Purpose of the Study:

To review recent advances in engineering microbial robustness and tolerance.
To introduce novel strategies for enhancing cell factory robustness.
To highlight systems and synthetic biology principles for holistic cell function engineering.

Main Methods:

Review of recent literature on microbial robustness and tolerance engineering.
Discussion of strategies including metabolic pathway balancing, transporter engineering, and adaptive laboratory evolution.
Emphasis on integrating systems and synthetic biology for improved cell factory performance.

Main Results:

Engineered microbes with robust phenotypes are essential for high yield and productivity.
Strategies discussed aim to overcome limitations imposed by industrial conditions and toxic compounds.
Adaptive laboratory evolution and transporter engineering are key approaches to enhance tolerance.

Conclusions:

Engineering robustness is critical for the industrial viability of microbial cell factories.
A holistic approach integrating systems and synthetic biology is needed for optimal cell function.
Further research into enhancing microbial tolerance will drive advancements in sustainable bioproduction.