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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Updated: Feb 23, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

A non-destructive DNA sampling technique for herbarium specimens.

Lara D Shepherd¹

¹Museum of New Zealand Te Papa Tongarewa, Wellington, New Zealand.

|September 1, 2017

Summary

A new non-destructive DNA extraction method for herbarium specimens preserves valuable plant samples. This technique successfully retrieves DNA from older herbarium material, advancing plant research without sample degradation.

Area of Science:

Botany
Molecular Biology
Conservation Genetics

Background:

Herbarium specimens are crucial genetic resources for plant research.
Existing DNA extraction methods are destructive, risking irreplaceable specimens like rare or type material.
A non-destructive approach is needed to preserve historical and rare plant collections.

Purpose of the Study:

To develop and present the first non-destructive DNA sampling method for herbarium specimens.
To enable DNA extraction without compromising the integrity of valuable herbarium collections.

Main Methods:

Development of a novel, non-destructive sampling technique for DNA extraction.
Application of the method to various herbarium specimens, focusing on leaves and stems.

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Analysis of DNA quality and quantity retrieved from aged specimens.

Main Results:

Successful DNA retrieval was achieved using the non-destructive method.
DNA was extracted from herbarium specimens up to 73 years old.
The method proved effective on robust leaf and stem tissues.

Conclusions:

The presented non-destructive method allows for DNA extraction from herbarium specimens.
This technique safeguards irreplaceable plant material for future research and conservation.
It opens new avenues for genetic studies using historical herbarium collections.