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Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
Published on: April 22, 2019
The effect of wool hydrolysates on squamous cell carcinoma cells in vitro. Possible implications for cancer treatment
Tatsiana Damps1, Anna Katarzyna Laskowska1, Tomasz Kowalkowski2,3
1Department of Neuropeptides, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
Abstract:
Squamous cell carcinoma of the skin is the second most common cutaneous malignancy. Despite various available treatment methods and advances in noninvasive diagnostic techniques, the incidence of metastatic cutaneous squamous cell carcinoma is rising. Deficiency in effective preventive or treatment methods of transformed keratinocytes leads to necessity of searching for new anticancer agents. The present study aims to evaluate the possibility of using wool hydrolysates as such agents. Commercially available compounds such as 5-fluorouracil, ingenol mebutate, diclofenac sodium salt were also used in this study. The process of wool degradation was based on chemical pre-activation and enzymatic digestion of wool. The effect of mentioned compounds on cell viability of squamous carcinoma cell line and healthy keratinocytes was evaluated. The obtained data show a significantly stronger effect of selected wool hydrolysates compared to commercial compounds (p<0.05) on viability of cells. The wool hydrolysates decreased squamous cell carcinoma cells viability by up to 67% comparing to untreated cells. These results indicate bioactive properties of wool hydrolysates, which affect the viability of squamous carcinoma cells and decrease their number. We hypothesize that these agents may be used topically for treatment of transformed keratinocytes in actinic keratosis and invasive squamous skin cancer in humans.
Insights
New research explores wool hydrolysates as potential treatments for skin cancer. These natural compounds show significant promise in reducing squamous cell carcinoma viability, offering a novel approach to treating transformed keratinocytes.
Area of Science:
- Dermatology
- Biochemistry
- Oncology
Background:
- Cutaneous squamous cell carcinoma (cSCC) is a prevalent skin cancer with rising metastatic rates.
- Current treatments face limitations, necessitating novel therapeutic strategies for transformed keratinocytes.
- There is a need for effective preventive and treatment methods against skin malignancies.
Purpose of the Study:
- To investigate the potential of wool hydrolysates as anticancer agents against skin cancer.
- To compare the efficacy of wool hydrolysates with established treatments like 5-fluorouracil, ingenol mebutate, and diclofenac sodium salt.
- To evaluate the impact of wool hydrolysates on the viability of squamous cell carcinoma and healthy keratinocytes.
Main Methods:
- Wool degradation via chemical pre-activation and enzymatic digestion.
- In vitro evaluation of wool hydrolysates and commercial compounds on cancer cell lines.
- Assessment of cell viability in squamous carcinoma cells and healthy keratinocytes.
Main Results:
- Wool hydrolysates demonstrated a significantly stronger effect on cell viability compared to commercial compounds (p<0.05).
- Selected wool hydrolysates reduced squamous cell carcinoma cell viability by up to 67% compared to untreated controls.
- The study identified bioactive properties in wool hydrolysates affecting cancer cell viability.
Conclusions:
- Wool hydrolysates exhibit significant bioactive properties against squamous cell carcinoma cells.
- These findings suggest potential topical applications for wool hydrolysates in treating actinic keratosis and invasive squamous skin cancer.
- Wool hydrolysates represent a promising new avenue for managing transformed keratinocytes and skin malignancies.
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