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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Allergic FcεRI- and pseudo-allergic MRGPRX2-triggered mast cell activation routes are independent and inversely

M Babina1, S Guhl1, M Artuc1

  • 1Department of Dermatology and Allergy, Allergy Center Charité, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.

Allergy
|September 1, 2017
PubMed

Insights

Allergic and pseudo-allergic mast cell (MC) degranulation pathways are independent, with no predictive value between them. Stem cell factor selectively dampens pseudo-allergic responses while promoting allergic ones.

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • Mast cells (MCs) mediate allergic reactions via FcεRI aggregation.
  • A pseudo-allergic pathway involving MRGPRX2 has been identified.
  • Interindividual variability exists in MC responses.

Purpose of the Study:

  • To investigate interindividual variability in skin MC degranulation.
  • To compare FcεRI-mediated allergic and MRGPRX2-mediated pseudo-allergic pathways.
  • To explore the role of stem cell factor (SCF) in these pathways.

Main Methods:

  • Examined skin MC responses to FcεRI and MRGPRX2 stimulation.
  • Assessed degranulation triggered by compound 48/80 and substance P.
  • Quantified MRGPRX2 mRNA expression variability.
  • Investigated the effect of SCF on both pathways.

Main Results:

  • FcεRI and MRGPRX2 pathways are independent, with no cross-predictive value.
  • Degranulation by compound 48/80 correlates with substance P-induced degranulation.
  • MRGPRX2 mRNA exhibits significant population-based variability.
  • SCF inhibits pseudo-allergic degranulation but promotes FcεRI-mediated degranulation.

Conclusions:

  • SCF exhibits selective MC-dampening functions.
  • Individual reactivity in one MC degranulation pathway does not predict reactivity in the other.
  • Understanding these distinct pathways is crucial for managing allergic and pseudo-allergic conditions.
Keywords:
MRGPRX2mast cellpseudo-allergystem cell factorsubstance P

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