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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Matrix-Assisted Laser Desorption Ionization (MALDI)

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Shotgun Lipidomics of Rodent Tissues
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Mass-spectrometry-based lipidomics.

Ting Hu1, Jin-Lan Zhang1

  • 1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, PR China.

Journal of Separation Science
|September 1, 2017
PubMed
Summary
This summary is machine-generated.

Lipidomics uses mass spectrometry to analyze lipids, crucial for cell functions and disease biomarkers. This review covers advancements in lipid extraction, analysis, and novel techniques like single-cell lipidomics.

Keywords:
lipidomicsliquid chromatographymass spectrometrysample pretreatment

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Systems Biology

Background:

  • Lipids are vital for cellular processes, energy storage, and signaling due to their diverse properties.
  • Lipidomics offers insights into lipid functions and disease biomarker discovery.
  • Mass spectrometry is the primary tool for lipid profiling and quantification.

Purpose of the Study:

  • To review the field of mass-spectrometry-based lipidomics.
  • To discuss recent advancements in lipid extraction, separation, and analysis.
  • To highlight novel lipidomics technologies and their applications.

Main Methods:

  • Mass spectrometry-based analytical techniques for lipid profiling.
  • Advanced lipid extraction and separation strategies.
  • Statistical analysis for interpreting lipidomic data.

Main Results:

  • Comprehensive overview of mass spectrometry applications in lipidomics.
  • Discussion of progress in key lipidomics methodologies.
  • Exploration of new techniques such as single-cell and flux-based lipidomics.

Conclusions:

  • Mass spectrometry-based lipidomics is essential for understanding lipid roles in physiology and disease.
  • Continued technological innovation is expanding the scope and precision of lipidomics.
  • Emerging lipidomics approaches promise deeper biological insights and improved diagnostics.