Related Experiment Video

Updated: Feb 23, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.0K

High-Quality Draft Genome Sequences for Four Drug-Resistant or Outbreak-Associated Shigella sonnei Strains Generated

Rebecca L Lindsey1, Dhwani Batra2, Lori Rowe2

  • 1Centers for Disease Control and Prevention, Atlanta, Georgia, USA Rebecca.Lindsey@cdc.hhs.gov.

Genome Announcements
|September 2, 2017
PubMed

Insights

Drug-resistant Shigella sonnei is a growing concern. This study presents whole-genome sequences of four outbreak isolates, revealing antibiotic resistance patterns crucial for public health strategies.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Drug-resistant Shigella sonnei presents a significant clinical and public health challenge.
  • Understanding the genetic basis of resistance is crucial for effective treatment and control.

Purpose of the Study:

  • To perform whole-genome sequencing on outbreak-associated Shigella sonnei isolates.
  • To characterize the antibiotic resistance profiles of these isolates.

Main Methods:

  • High-quality draft whole-genome sequencing was performed.
  • Isolates were analyzed for antibiotic resistance.

Main Results:

  • Four outbreak-associated Shigella sonnei isolates were sequenced.
  • Three isolates exhibited resistance to two or more antibiotics.
  • One isolate showed resistance to streptomycin exclusively.

Conclusions:

  • Genomic data provides insights into the evolution and spread of drug-resistant Shigella sonnei.
  • Characterization of resistance mechanisms is vital for developing targeted interventions.

More Related Videos

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow10:44

Generating Whole Bacterial Genomes from Clinical Samples using a Target Enrichment Workflow

Published on: August 15, 2025

1.2K
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.06:55

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

Published on: November 7, 2018

9.5K

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
99.3K
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
775.7K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
759
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.2K