Related Experiment Video
Updated: Feb 23, 2026
09:23
Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model
Published on: July 29, 2012
14.5K
Long intergenic non-coding RNA GALMD3 in chicken Marek's disease
Bo Han1, Yanghua He2, Li Zhang3
1Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Scientific Reports
|September 2, 2017
Summary
This study identifies a long non-coding RNA, linc-GALMD3, highly expressed in Marek
Area of Science:
- Molecular Biology
- Virology
- Immunology
Background:
- Long intergenic non-coding RNAs (lincRNAs) are implicated in various diseases, including cancers and neurological disorders.
- Marek's disease (MD) is a significant T-cell lymphoid neoplasia in chickens caused by Marek's disease virus (MDV).
- Aberrant lincRNA expression is linked to disease pathogenesis, necessitating investigation into their roles in MDV infection.
Purpose of the Study:
- To identify and characterize lincRNAs involved in Marek's disease pathogenesis.
- To elucidate the regulatory functions of identified lincRNAs in MDV-infected cells.
- To explore the potential of lincRNAs as biomarkers or therapeutic targets for Marek's disease.
Main Methods:
- RNA sequencing (RNA-Seq) to identify differentially expressed lincRNAs in MDV-infected cells.
- Quantitative reverse transcription PCR (qRT-PCR) for validation of lincRNA expression.
- Loss-of-function studies using shRNA to investigate the regulatory roles of linc-GALMD3.
Main Results:
- linc-GALMD3 was identified and validated as highly expressed in MDV-infected CD4+ T cells.
- Knockdown of linc-GALMD3 affected downstream gene expression, including gga-miR-223, and impacted mitochondrial function and cell cycle.
- MDV replication was significantly repressed upon linc-GALMD3 knockdown in chicken embryo fibroblast (CEF) cells.
Conclusions:
- linc-GALMD3 plays a critical regulatory role in chicken Marek's disease.
- linc-GALMD3 influences host gene expression related to mitochondrial structure, cell cycle, and viral replication.
- linc-GALMD3 represents a promising candidate biomarker for MD prevention, diagnosis, and treatment.
Related Concept Videos
02:32Exon Recombination
4.2K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
4.2K
03:18Non-LTR Retrotransposons
13.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.5K
02:28Leaky Scanning
5.8K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.8K