Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

9.0K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differences in P-Type and Type 1 Uropathogenic <i>Escherichia coli</i> Urinary Anti-Adhesion Activity of Cranberry Fruit Juice Dry Extract Product and D-Mannose Dietary Supplement.

Journal of dietary supplements·2024
Same author

Improved <i>in vitro</i> Hemagglutination Assays Utilizing P-Type and Type 1 Uropathogenic <i>Escherichia coli</i> to Evaluate Bacterial Anti-Adhesion Activity of Cranberry Products.

Journal of dietary supplements·2023
Same author

Cranberry proanthocyanidins composite electrospun nanofibers as a potential alternative for bacterial entrapment applications.

Journal of biomedical materials research. Part B, Applied biomaterials·2022
Same author

Synthesis of Fluorescent Proanthocyanidin-Cinnamaldehydes Pyrylium Products for Microscopic Detection of Interactions with Extra-Intestinal Pathogenic <i>Escherichia coli</i>.

Journal of agricultural and food chemistry·2021
Same author

Cranberry Proanthocyanidins-PANI Nanocomposite for the Detection of Bacteria Associated with Urinary Tract Infections.

Biosensors·2021
Same author

Identification of A-Type Proanthocyanidins in Cranberry-Based Foods and Dietary Supplements by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, First Action Method: 2019.05.

Journal of AOAC International·2021

Related Experiment Video

Updated: Feb 23, 2026

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

12.1K

Cranberry Proanthocyanidins - Protein complexes for macrophage activation.

Sergio M Carballo1, Linda Haas, Christian G Krueger

  • 1Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr, Madison, WI 53706, USA. jdreed@wisc.edu.

Food & Function
|September 2, 2017
PubMed
Summary

Cranberry proanthocyanidins (PAC) form stable complexes with proteins like BSA and HEL, modulating macrophage immune responses. These PAC-protein complexes influence macrophage activation and antigen presentation, suggesting potential roles in innate and acquired immunity.

More Related Videos

Proteomic Profiling of Macrophages by 2D Electrophoresis
07:53

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

13.4K
In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
12:27

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages

Published on: December 30, 2017

14.1K

Related Experiment Videos

Last Updated: Feb 23, 2026

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

12.1K
Proteomic Profiling of Macrophages by 2D Electrophoresis
07:53

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

13.4K
In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages
12:27

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages

Published on: December 30, 2017

14.1K

Area of Science:

  • Food Science and Nutrition
  • Immunology
  • Biochemistry

Background:

  • Cranberry proanthocyanidins (PAC) are bioactive compounds with potential health benefits.
  • Understanding the interaction of PAC with proteins is crucial for their biological activity.
  • Macrophage activation and antigen presentation are key components of the immune system.

Purpose of the Study:

  • To characterize the interaction between cranberry PAC and model proteins (BSA, HEL).
  • To determine the effects of these PAC-protein complexes on macrophage activation.
  • To investigate the impact of PAC-protein complexes on antigen presentation.

Main Methods:

  • Isolation of PAC from cranberry.
  • Complexation of PAC with bovine serum albumin (BSA) and hen egg-white lysozyme (HEL).
  • Characterization using MALDI-TOF MS, gel electrophoresis, and zeta-potential measurements.
  • Assessment of macrophage activation (COX-2, iNOS expression) and antigen presentation (IL-2 production) in vitro.
  • Fluorescence microscopy for studying endocytosis.

Main Results:

  • Stable PAC-protein complexes were formed, altering protein structure without precipitation.
  • PAC-BSA complexes reduced inflammatory markers (COX-2, iNOS) in LPS-stimulated macrophages.
  • PAC-HEL complexes enhanced antigen uptake, processing, and presentation, leading to increased IL-2 production.
  • Macrophages endocytosed PAC-BSA complexes.

Conclusions:

  • Cranberry PAC-protein complexes are stable and can modulate macrophage immune functions.
  • These complexes show potential in influencing both innate (activation) and acquired (antigen presentation) immune responses.
  • PAC-protein interactions offer a mechanism for cranberry bioactives to impact immunity.