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Expression of Heterologous PrP and Prion Propagation in RK13 Cells
Zaira E Arellano-Anaya1,2, Alvina Huor1,2, Pascal Leblanc3
1INRA, UMR 1225, IHAP, 31076, Toulouse, France.
Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2017
Summary
This study engineered RK13 cells to express prion proteins, enabling chronic prion infection and establishing a model for prion disease research. These cell lines are crucial for studying prion propagation and developing titration methods.
Area of Science:
- Cell biology
- Neuroscience
- Infectious diseases
Background:
- Cultured cells are vital for studying prion infections.
- Most cell lines resist prion propagation.
- RK13 cells are a rare exception, susceptible to prion infection.
Purpose of the Study:
- To develop RK13 cell clones that inducibly express heterologous prion protein (PrP).
- To establish chronically prion-infected cell cultures.
- To select cell clones for prion titration.
Main Methods:
- Genetic engineering of RK13 cells for inducible PrP expression.
- Establishment and maintenance of chronically prion-infected cultures.
- Selection of suitable cell clones for prion titration.
Main Results:
- Generated stable RK13 cell clones with inducible heterologous PrP expression.
- Established chronically prion-infected cell cultures.
- Identified cell clones appropriate for cell-based prion titration.
Conclusions:
- Inducible PrP expression in RK13 cells facilitates prion infection studies.
- These engineered cells provide a robust model for prion propagation research.
- The selected clones are valuable tools for prion titration assays.
