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Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Feb 23, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Lectin-Array Blotting.

Raquel Pazos¹, Juan Echevarria¹, Alvaro Hernandez¹

¹Glycotechnology Laboratory, CIC biomaGUNE, San Sebastian, Spain.

Current Protocols in Cell Biology

|September 2, 2017

Summary

This study introduces a rapid glycoproteomics method for analyzing protein glycosylation in complex mixtures. It enables detailed glycan biomarker discovery by profiling individual proteins separated by gel electrophoresis.

Keywords:

electrophoresis glycosylation lectins microarray

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Last Updated: Feb 23, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

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Published on: May 8, 2014

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Area of Science:

Biochemistry
Molecular Biology
Glycobiology

Background:

Aberrant protein glycosylation is a key indicator in diseases like cancer and neurodegeneration.
Discovering glycan biomarkers requires efficient and unbiased glycoproteomics techniques.

Purpose of the Study:

To develop a facile and rapid protocol for qualitative analysis of protein glycosylation.
To enable individual glycan binding profiling of separated proteins, overcoming limitations of traditional methods.

Main Methods:

Proteins are separated by gel electrophoresis and transferred directly to a glass slide with printed lectin arrays.
Fluorescently labeled glycoproteins bind to specific lectins via carbohydrate-lectin interactions.
Binding profiles are analyzed using a fluorescent scanner, analyzing interactions with up to 20 lectin probes.

Main Results:

The method provides individual lectin binding profiles for all separated proteins, unlike average signals from traditional lectin arrays.
It avoids protein excision from gels, streamlining the analysis process.
The technique generates data equivalent to 20 lectin blots in a single experiment, offering detailed epitope insights.

Conclusions:

This novel method offers a rapid and detailed approach for glycan biomarker discovery.
It enhances the understanding of protein glycosylation patterns in complex biological samples.
The technique holds significant potential for disease marker identification and research.