Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 23, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K

Cellular Detection of G-Quadruplexes by Optical Imaging Methods.

Souheila Amor1, Sunny Y Yang2, Judy M Y Wong2

  • 1Institut de Chimie Moléculaire, ICMUB CNRS UMR6302, UBFC Dijon, France.

Current Protocols in Cell Biology
|September 2, 2017
PubMed
Summary

Researchers developed novel fluorescent probes, TASQ, to visualize G-quadruplexes (G4s) in cells. These probes enable advanced imaging techniques, aiding the study of G4s in gene regulation and diseases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex-linked helicases DDX3X and DDX3Y regulate G-quadruplex-associated stress in neurons.

Cell death & disease·2026
Same author

Epigenetic control of telomeric RNA maintains heterochromatin in telomerase-driven cancers.

Signal transduction and targeted therapy·2026
Same author

1950-2000: five decades of curiosity-driven discovery in alternative DNA structures.

Nucleic acids research·2026
Same author

DNA damaging properties of G-quadruplex ligand QN-302 are potentiated by the DNA repair inhibitor Olaparib and mitigated by the molecular helicase PhpC.

Genome biology·2026
Same author

Designing an ecofriendly catalyst for a sustainable use of water resources.

National science review·2026
Same author

Photoactivatable, Biomimetic Ligand photoMultiTASQ Traps DNA/RNA G‑Quadruplexes and Their Protein Binding Partners.

ACS bio & med chem Au·2025

Area of Science:

  • Chemical Biology
  • Molecular Biology
  • Genetics

Background:

  • G-quadruplexes (G4s) are crucial nucleic acid structures involved in gene regulation.
  • G4s play roles in transcription, translation, chromosomal stability, and disease pathogenesis.

Purpose of the Study:

  • To present protocols for visualizing G-quadruplexes within cells.
  • To introduce a novel class of G4-selective fluorophores, TASQ ligands.

Main Methods:

  • Development of template-assembled synthetic G-quartet (TASQ) ligands.
  • Application of TASQ probes for imaging in fixed and live cells.
  • Utilizing multiphoton microscopy, confocal microscopy, and real-time fluorescent imaging.

Main Results:

Keywords:
G-quadruplexfluorescencelive cell imagingmicroscopysmart probes

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.7K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.9K

Related Experiment Videos

Last Updated: Feb 23, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.3K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.7K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.9K
  • TASQ ligands demonstrate versatility for various cellular imaging techniques.
  • Successful visualization of G-quadruplexes in cellular environments.
  • Enabling detailed study of G4 involvement in cellular mechanisms.

Conclusions:

  • TASQ probes offer a powerful tool for G-quadruplex research.
  • Advanced imaging of G4s facilitates understanding of their roles in biological processes and diseases.
  • This work provides essential protocols for the scientific community.