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Related Experiment Video

Updated: Feb 23, 2026

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

Novel luciferase-opsin combinations for improved luminopsins.

Sung Young Park¹, Sang-Ho Song^2,3, Brandon Palmateer^4,5

¹Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul, Republic of Korea.

Journal of Neuroscience Research

|September 2, 2017

Summary

New luminopsins combine high-light emitting luciferases with channelrhodopsins for advanced neuronal control. These tools enable bimodal optogenetic and chemogenetic manipulation for brain function analysis.

Keywords:

6-OHDA rat GLucM23 bioluminescence chemogenetics iChloC optogenetics

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Building a Simple and Versatile Illumination System for Optogenetic Experiments

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Last Updated: Feb 23, 2026

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Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

Area of Science:

Neuroscience
Molecular Biology
Biotechnology

Background:

Luminopsins, fusions of luciferase and opsin, offer genetically encoded neuronal control.
Existing luminopsin tools have limitations in versatility and light emission.

Purpose of the Study:

To enhance luminopsin tools by incorporating a high light-emission luciferase variant (Gaussia luciferase mutant GLucM23).
To develop novel luminopsins for bimodal optogenetic and chemogenetic neuronal manipulation.

Main Methods:

Fusion of GLucM23 with depolarizing (Volvox channelrhodopsin-1) and hyperpolarizing (iChloC) channelrhodopsins.
Activation of channelrhodopsins using luciferase substrate.
Measurement of neuronal responses in single neurons and populations in mice and rats.
Behavioral analysis of amphetamine-induced rotations in rats.

Main Results:

Successful creation of LMO4 and iLMO4 luminopsins with enhanced light sensitivity.
Efficient channelrhodopsin activation demonstrated in vitro and in vivo.
Significant changes in male rat behavior observed, indicating functional manipulation.

Conclusions:

The novel luminopsins (LMO4, iLMO4) expand the toolkit for neuronal manipulation.
These tools facilitate bimodal optogenetic and chemogenetic analyses of brain function.
The enhanced light emission and sensitivity offer improved experimental capabilities.