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Updated: Feb 23, 2026

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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The Bioengineered Cardiac Left Ventricle.

Nikita M Patel1, Ravi K Birla

  • 1From the Department of Biomedical Engineering, University of Houston, Houston, Texas.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|September 2, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a bioengineered complete ventricle (BECV) and trileaflet valve (BETV) to potentially treat hypoplastic left heart syndrome (HLHS). This novel cardiac organoid fabrication offers a foundation for engineered left ventricle development.

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Cardiovascular Research

Background:

  • Hypoplastic left heart syndrome (HLHS) involves underdeveloped left ventricles and aortic valves, currently treated by right ventricle refunctionalization.
  • Cardiac organoids offer simplified, flow-looped chambers for ventricle function research.
  • Developing a complete bioengineered ventricle represents a significant advancement in cardiac organoid technology.

Purpose of the Study:

  • To establish the foundational methods for fabricating a bioengineered complete ventricle (BECV).
  • To develop a bioengineered trileaflet valve (BETV) mimicking neonate aortic valve geometry.
  • To create a functional cardiac construct for potential HLHS treatment.

Main Methods:

  • Fabrication of bioengineered trileaflet valve (BETV) molds and chitosan scaffolds.
  • Assembly of BECV by integrating BETV into a bioengineered open ventricle (BEOV).
  • Two-stage cellularization of the BEOV and passive seeding of BETV with cardiac fibroblasts, followed by perfusion culture.

Main Results:

  • The fabricated BECV generated an average pressure of 0.06–0.12 mm Hg and a biopotential output of 1.02 mV.
  • Histologic assessment revealed cardiomyocyte aggregates on the BECV surface.
  • BETV showed randomly distributed cardiac fibroblasts.

Conclusions:

  • The study presents a novel method for fabricating a bioengineered complete ventricle (BECV).
  • This BECV fabrication provides a basis for developing a functional engineered left ventricle.
  • The developed construct holds potential for clinical applications in treating hypoplastic left heart syndrome (HLHS).