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Related Experiment Video

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Regulation of Trophic Factor Expression by Innervating Target Regions in Intraocular Double Transplants.

L Willis1, E M Quintero1, M Nelson1

  • 1Department of Physiology and Neuroscience and the Center on Aging, Medical University of South Carolina, Charleston, SC 29425.

Cell Transplantation
|September 3, 2017
PubMed
Summary

Brain-derived neurotrophic factor (BDNF) expression in the hippocampus and brain stem is regulated by neural connections. Contact between these tissues in vivo influences BDNF levels, suggesting retrograde transport mechanisms.

Keywords:
BDNFHippocampal formationLocus coeruleusNeurotrophinsTransplantation

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Trophic factors are crucial for neural survival and function.
  • Regulation of growth factor expression and cell-cell interactions in the nervous system are not well understood.
  • Brain-derived neurotrophic factor (BDNF) influences noradrenergic innervation of the hippocampus.

Purpose of the Study:

  • To investigate if neural connections between locus coeruleus (LC) noradrenergic neurons and the hippocampus affect BDNF expression.
  • To determine the role of innervation and target tissue contact in regulating BDNF levels.

Main Methods:

  • Fetal rat brain stem (LC) and hippocampal tissues were co-transplanted or transplanted individually into the anterior chamber of the eye.
  • Tissues were grown for 6 weeks.
  • BDNF protein levels were quantified using ELISAs.
  • Colchicine treatment was used to assess axonal transport.

Main Results:

  • Ocular transplantation increased hippocampal BDNF levels compared to in vivo controls.
  • Co-grafting (hippocampus and brain stem) significantly elevated BDNF levels in both tissues compared to single grafts.
  • Triple grafts further increased BDNF levels.
  • Colchicine treatment reduced hippocampal BDNF but only partially reduced brain stem BDNF, suggesting retrograde transport.

Conclusions:

  • Appropriate hippocampal innervation or target tissue contact is essential for regulating BDNF expression in the brain stem.
  • Retrograde transport of BDNF occurs between connected fetal tissues grafted in the eye.