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Antiglycation activity of melatonin.

A E Dontsov1, N D Vospelnikova2, P P Zack3

  • 1Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia. adontsovnick@yahoo.com.

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The hormone melatonin demonstrates antiglycation activity by slowing Schiff adduct formation in vitro. Melatonin specifically inhibits the Maillard reaction but not BSA modification by reactive carbonyls like methylglyoxal.

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Area of Science:

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Background:

  • The Maillard reaction, a non-enzymatic glycation process, contributes to aging and diabetic complications.
  • Advanced glycation end products (AGEs) form through reactions between sugars and proteins.
  • Melatonin, a hormone regulating sleep, is also studied for its antioxidant and anti-inflammatory properties.

Purpose of the Study:

  • To investigate the antiglycation activity of melatonin in vitro.
  • To determine melatonin's effect on specific glycation pathways, including the Maillard reaction and modification by reactive carbonyls.

Main Methods:

  • In vitro assays were used to study the interaction between melatonin, bovine serum albumin (BSA), and sugars (fructose, methylglyoxal).
  • Accumulation of fluorescent Schiff adducts, an indicator of glycation, was measured.
  • The effect of melatonin on BSA modification by fructose and methylglyoxal was compared.

Main Results:

  • Melatonin significantly inhibited the accumulation of fluorescent Schiff adducts during BSA modification with fructose.
  • Melatonin did not affect the modification of BSA by methylglyoxal, a reactive carbonyl compound.
  • These findings suggest melatonin's specific inhibitory effect on the Maillard reaction.

Conclusions:

  • Melatonin exhibits antiglycation activity in vitro, specifically targeting the Maillard reaction.
  • Melatonin's inhibitory effect is dependent on the glycation pathway, as it does not impact BSA modification by methylglyoxal.
  • Further research into melatonin's role in mitigating AGEs formation is warranted.