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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
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Exploring gene expression changes in the amphioxus gill after poly(I:C) challenge using digital expression profiling.

Qi-Lin Zhang1, Han-Yue Qiu1, Ming-Zhong Liang2

  • 1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210023 China.

Fish & Shellfish Immunology
|September 4, 2017
PubMed
Summary
This summary is machine-generated.

Amphioxus gills show significant gene expression changes in response to viral mimic poly(I:C) (pIC). This study reveals key genes involved in antiviral defense, offering insights into vertebrate gill immunity evolution.

Keywords:
Branchiostoma belcheriDigital gene expressionGillPoly (I:C)

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Area of Science:

  • * Evolutionary immunology
  • * Cephalochordate research
  • * Comparative genomics

Background:

  • * Amphioxus (Branchiostoma belcheri) is a crucial model for understanding vertebrate immune evolution.
  • * Previous studies noted microRNA (miRNA) changes in amphioxus gills post-immune stimulation.
  • * Gene expression responses to immune stress in amphioxus gills remain largely unexplored.

Purpose of the Study:

  • * To investigate gene expression profiles in amphioxus gills upon viral mimic exposure.
  • * To identify differentially expressed genes (DEGs) and their associated functions.
  • * To understand the role of amphioxus gill immunity in vertebrate evolution.

Main Methods:

  • * High-throughput RNA sequencing (RNA-seq) for tag-based digital gene expression (DGE) profiling.
  • * Analysis of six libraries from control and poly(I:C) (pIC)-exposed amphioxus gills (three biological replicates per group).
  • * Validation of RNA-seq data using real-time quantitative PCR (qRT-PCR).

Main Results:

  • * 1999 differentially expressed genes (DEGs) identified: 571 upregulated and 1428 downregulated.
  • * Enrichment analysis linked DEGs to immune response, apoptosis, cancer, and metabolic processes.
  • * Eight key genes (TRADD, MARCH, RNF31, NF-κb, CYP450, TNFRSF6B, IFI, LECT1) were confirmed to be induced in the antiviral response.

Conclusions:

  • * This study provides a comprehensive gene expression dataset for amphioxus gills under viral challenge.
  • * Identified DEGs and key antiviral genes offer insights into amphioxus antiviral immunity.
  • * Findings contribute to understanding the evolutionary trajectory of gill immunity in vertebrates.