Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thoughts and Therapies: Melanoma Brain Metastases.

Cells·2026
Same author

Engineering function in lung biology: integrating imaging, regenerative constructs, and functional biodesign.

American journal of physiology. Lung cellular and molecular physiology·2026
Same author

Vascular Endothelial Growth Factor-D Improves Lung Vascular Integrity During Acute Lung Injury.

Circulation research·2026
Same author

Reply: Model Choice and Interpretation in Coronary Tissue Engineering: Still More Questions Than Answers.

JACC. Basic to translational science·2026
Same author

Long-term safety and efficacy outcomes of the Acellular Tissue Engineered Vessel (ATEV) in extremity arterial trauma repair.

Journal of vascular surgery cases and innovative techniques·2025
Same author

Short-term performance of Symvess (acellular tissue engineered vessel-tyod) compared to external control data for autologous vein in treatment of extremity arterial injury.

Trauma surgery & acute care open·2025

Related Experiment Video

Updated: Feb 23, 2026

Preparation of Decellularized Kidney Scaffolds in Rats
06:09

Preparation of Decellularized Kidney Scaffolds in Rats

Published on: March 18, 2021

4.8K

Decellularized Native and Engineered Arterial Scaffolds for Transplantation.

Shannon L M Dahl1, Jennifer Koh2, Vikas Prabhakar3

  • 1Departments of Biomedical Engineering, Durham, NC 27708.

Cell Transplantation
|September 5, 2017
PubMed
Summary

Researchers optimized decellularization methods for vascular grafts, improving cell removal while preserving mechanical integrity. This advance supports the use of engineered arteries as immediate grafts or scaffolds for cell transplantation.

Keywords:
ArteriesDecellularizationExtracellular matrixScaffoldsTissue engineering

More Related Videos

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

12.7K
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

8.9K

Related Experiment Videos

Last Updated: Feb 23, 2026

Preparation of Decellularized Kidney Scaffolds in Rats
06:09

Preparation of Decellularized Kidney Scaffolds in Rats

Published on: March 18, 2021

4.8K
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
13:04

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

12.7K
Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

8.9K

Area of Science:

  • Biomaterials Science
  • Vascular Tissue Engineering
  • Regenerative Medicine

Background:

  • Coronary artery bypass grafting demands numerous small-diameter vascular grafts annually.
  • Current tissue engineering and cadaveric options face limitations like long production times, limited availability, and disease transmission risks.
  • Decellularized engineered arteries offer potential for immediate implantation, cell seeding, or cell delivery scaffolds.

Purpose of the Study:

  • To evaluate the impact of decellularization on the matrix and mechanical properties of vascular tissue.
  • To compare cellular elimination, extracellular matrix retention, and mechanical characteristics of porcine carotid arteries using three decellularization methods.
  • To investigate the decellularization of tissue-engineered arteries and their subsequent use as scaffolds for cell seeding.

Main Methods:

  • Porcine carotid arteries were subjected to three different decellularization protocols.
  • Tissue-engineered arteries were decellularized for the first time.
  • Decellularized native arteries were utilized as scaffolds for vascular cell seeding.

Main Results:

  • A decellularization method was identified that effectively removed cells from both native and engineered arteries.
  • This method minimized compromise to the mechanical integrity of the vascular grafts.
  • Successful decellularization of engineered tissues and reseeding with vascular cells were demonstrated.

Conclusions:

  • Optimized decellularization techniques can yield vascular grafts with preserved mechanical properties.
  • Decellularized engineered arteries are a viable option for vascular reconstruction.
  • These findings support the use of decellularized engineered arteries as scaffolds for cell transplantation and immediate implantation.