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Updated: Feb 23, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Molecular and phenotypic characterization of endophytic bacteria isolated from sulla nodules
Hamida Beghalem1, Khadidja Aliliche2, Ali Chriki2
1Laboratory of Biochemistry and Molecular Biology, Code UR13ES34 Research Unit, Carthage University, Faculty of Sciences of Bizerte, Zarzouna, Bizerte, Tunisia.
Abstract:
In the current study, bacterial diversity was investigated in root nodules of Sulla pallida and Sulla capitata. The isolates were analyzed on the basis of their phenotypic and molecular characteristics. The phylogenetic analysis based on 16S rRNA and housekeeping genes (recA and atpD) showed that the isolated bacteria related to Sinorhizobium, Neorhizobium, Phyllobacterium, Arthrobacter, Variovorax and Pseudomonas genera. This is the first report of Neorhizobium genus associated with Hedysarum genus. Phenotypically, all strains tolerate the elevated temperature of 40 °C, and salt stress at a concentration of 2%. In addition, the isolates failed to induce nodulation on their original host; and the symbiotic genes could not be amplified, suggesting that these strains are endophytic bacteria.
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