Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

GABA interaction with lipids in organic medium.

D Beltramo, S Kivatinitz, E Lassaga

    Life Sciences
    |August 10, 1987
    PubMed
    Summary

    Gamma-aminobutyric acid (GABA) and glutamate interact with certain lipids in a biphasic system, but not in an aqueous medium. This interaction lacks specificity and does not meet criteria for neurotransmitter receptors.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of microencapsulated ferrous sulfate particle size on Cheddar cheese composition and quality.

    Journal of dairy science·2018
    Same author

    Effectiveness of acellular pertussis vaccine and evolution of pertussis incidence in the community of Madrid from 1998 to 2015.

    Vaccine·2018
    Same author

    Correlation of three-liquid-phase equilibria involving ionic liquids.

    Physical chemistry chemical physics : PCCP·2016
    Same author

    Repeated oral co-exposure to yessotoxin and okadaic acid: a short term toxicity study in mice.

    Toxicon : official journal of the International Society on Toxinology·2013
    Same author

    Re-emergence of leishmaniasis in Spain: community outbreak in Madrid, Spain, 2009 to 2012.

    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2013
    Same author

    Differential findings regarding molecular epidemiology of tuberculosis between two consecutive periods in the context of steady increase of immigration.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2012

    Area of Science:

    • Neurochemistry
    • Biochemistry
    • Molecular Biology

    Background:

    • Gamma-aminobutyric acid (GABA) and glutamate are key neurotransmitters.
    • Understanding their interactions with cellular components is crucial for neuroscience.

    Purpose of the Study:

    • To investigate the interaction of GABA and glutamate with various lipids.
    • To determine if these interactions resemble neurotransmitter receptor binding.

    Main Methods:

    • Utilized a biphasic aqueous-organic partition system to study 3H-GABA and 14C-glutamate interactions with lipids.
    • Assessed interactions with specific lipids including sphingomyelin, phosphatidylcholine, and others.
    • Investigated displacement by other amino acids to assess specificity.

    Main Results:

    • 3H-GABA and 14C-glutamate interacted with several phospholipids and glycolipids in the organic phase, but not cholesterol or ceramide.
    • No interaction was observed in a homogeneous aqueous medium.
    • Interactions showed low affinity (millimolar Kd) and lacked pharmacological specificity, as demonstrated by displacement studies.
    • High lipid concentrations (>2 microM) were required for detection.

    Conclusions:

    • Lipids studied in a biphasic system do not meet classical criteria for GABA or glutamate neurotransmitter receptors.
    • The observed interactions are non-specific and require specific conditions (biphasic system, high lipid concentration).

    Related Experiment Videos