Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

EC array sensor concepts and data.

W R Matson, P G Gamache, M F Beal

    Life Sciences
    |August 17, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Multiparameter assays using coulometric electrode series array sensors and liquid chromatography (n-ELC) enhance metabolic pathway analysis for neurological disease research. This advanced technique improves resolution, enabling detailed studies of complex systems like the kynurenine pathway in Huntington's Disease.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Activation of sirtuin 1 as therapy for the peroxisomal disease adrenoleukodystrophy.

    Cell death and differentiation·2015
    Same author

    Impaired brain creatine kinase activity in Huntington's disease.

    Neuro-degenerative diseases·2010
    Same author

    Influence of mitochondrial enzyme deficiency on adult neurogenesis in mouse models of neurodegenerative diseases.

    Neuroscience·2008
    Same author

    Neuroprotective and neurorestorative strategies for neuronal injury.

    Neurotoxicity research·2006
    Same author

    Prospects for redox-based therapy in neurodegenerative diseases.

    Neurotoxicity research·2006
    Same author

    Retraction.

    Proceedings of the National Academy of Sciences of the United States of America·2006

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Neuroscience

    Background:

    • Metabolic pathway analysis is crucial for understanding neurological disorders and drug actions.
    • Conventional liquid chromatography (LC) has limitations in resolving complex biological samples.
    • Advanced sensor technology is needed to increase the resolving power of analytical methods.

    Purpose of the Study:

    • To introduce and validate the utility of coulometric electrode series array sensors coupled with liquid chromatography (n-ELC) for multiparameter metabolic pathway assays.
    • To demonstrate the enhanced resolving power of n-ELC compared to conventional LC.
    • To showcase the application of n-ELC in studying specific metabolic systems relevant to neurological diseases.

    Main Methods:

    • Development and optimization of coulometric electrode series array sensors for integration with computer-controlled LC systems.

    Related Experiment Videos

  • Implementation of both isocratic and gradient elution modes for n-ELC.
  • Establishment of a 56-component isocratic method for analyzing the kynurenine system.

    • Main Results:

    • n-ELC multiplies the resolving power of conventional LC by factors of 10 to 50.
    • Femtogram level potential time (ET) separations are achievable for multiple components.
    • A 56-component method successfully analyzed the kynurenine system, demonstrating analytical capabilities.

    Conclusions:

    • n-ELC represents a significant advancement in analytical chemistry for metabolic pathway studies.
    • This technology offers powerful tools for unraveling disease mechanisms and classifying neurological conditions.
    • The presented method provides a foundation for handling multiparameter databases in experimental design for neurological research.