DNA contents and molecular karyotypes of hybrid Trypanosoma brucei

We have used restriction fragment length polymorphism markers to characterise parental and hybrid trypanosome stocks. Unexpected differences in the intensities of Southern hybridisation banding patterns led us to suspect that the hybrid organisms contained more DNA than the parental stocks. This has been confirmed using flow cytofluorimetry (FCF). Hybrids contained significantly more DNA than the parents, both as procyclic organisms (1.5 fold) and as bloodstream forms (1.5-1.6 fold). The DNA contents of both forms were stable through prolonged culture (procyclics), or serial passage (bloodstream forms), although limited data indicated that falls in DNA content could occur in bloodstream forms. FCF analysis of purified nuclei revealed that the increased DNA content of hybrids could be wholly ascribed to nuclear DNA. Our methods are able to detect hybrid organisms with elevated DNA contents in uncloned isolates following cyclical mixed transmission. We have used alternating field electrophoresis techniques to investigate whether the inheritance by the hybrids of the smaller chromosomes could account for their elevated DNA contents. Hybrids lacked the single 500 kb chromosome from one of the parents but appeared to have virtually double the amount of minichromosomes. However, this increase could only account for about 20% of the additional DNA. We are unable at present to distinguish between models for hybrid formation based on the fusion of predominantly diploid cells, and models in which the diploid chromosomes participate in conventional meiosis.