High-performance liquid chromatography thermodynamic study of new potential antiepileptic compounds on a cholesterol
Jolanta Flieger1, Hanna Trębacz2, Magdalena Pizoń1
1Department of Analytical Chemistry, Medical University of Lublin, Lublin, Poland.
Abstract:
Basic thermodynamic functions responsible for retention of new 1,2,4-triazole derivatives exhibiting varied antiepileptic activity on cholesterol-based stationary phase were determined. Evaluation of the Gibbs energy change, the change in enthalpy and the change in entropy was based on the van't Hoff relationship representing lnk versus 1/T. A detailed discussion of the van't Hoff equation, exploring the influence of the phase ratio on deviations from linearity in a van't Hoff plot is presented. We show chromatographic evidence to the question of how a varied mobile phase composition may cause different thermodynamic phase ratios. The analysis of data from a differential scanning calorimetry excluded any phase transitions of either the individual solutes or cholesterol stationary phase suspended in the mobile phase components within the studied temperature range.
Related Concept Videos
High-Performance Liquid Chromatography: Elution Process
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion-Exchange Chromatography
High-Performance Liquid Chromatography: Types of Detectors
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation


