Related Experiment Video

Updated: Feb 23, 2026

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
09:01

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Published on: December 10, 2013

8.4K

Syncytia Induction by Clinical Isolates of Human Respiratory Syncytial Virus A

Talita Bianca Gagliardi1, Miriã Ferreira Criado, José Luiz Proença-Módena

  • 1Department of Cell Biology, University of São Paulo School of Medicine, Ribeirão Preto, Brazil.

Intervirology
|September 5, 2017
PubMed
Abstract

Insights

Syncytia formation and F protein mRNA levels are crucial for human respiratory syncytial virus (HRSV) progeny yield. Improved isolation methods enhance understanding of HRSV replication dynamics in clinical settings.

Area of Science:

  • Virology
  • Cell Biology

Background:

  • Human respiratory syncytial virus (HRSV) is a major pediatric respiratory pathogen.
  • Syncytia formation is a key characteristic of HRSV-induced cytopathic effects.
  • Understanding factors influencing HRSV replication is critical for developing effective treatments.

Purpose of the Study:

  • To report methodological improvements for primary HRSV isolation.
  • To investigate the role of syncytia formation in HRSV replication.
  • To determine the correlation between F protein mRNA levels and HRSV progeny yield.

Main Methods:

  • Isolation of HRSV strains A and B from nasopharyngeal aspirates using HEp-2 cell cultures.
  • Evaluation of syncytia formation using two distinct assays.
  • Quantification of F protein mRNA via real-time PCR and HRSV progeny titration by plaque assay.

Main Results:

  • Successful primary isolation of HRSV from 90.7% of samples.
  • Variations in syncytia quantity, size, and F protein mRNA levels among HRSV-A clinical isolates.
  • Direct correlation observed between F protein mRNA levels and HRSV progeny production.

Conclusions:

  • F protein mRNA levels are directly related to HRSV progeny production.
  • Syncytia quantity correlates with inoculum size but not directly with HRSV-A F protein mRNA levels.
  • Methodological advancements facilitate the study of HRSV replication and its clinical implications.
Keywords:
HRSV F proteinRespiratory syncytial virusRespiratory syncytial virus clinical isolatesSyncytia quantification

More Related Videos

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

17.3K
An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay09:14

An Improved and High Throughput Respiratory Syncytial Virus RSV Micro-neutralization Assay

Published on: January 26, 2019

11.6K

Related Concept Videos

Acute Pharyngitis01:30

Acute Pharyngitis

Introduction
Acute pharyngitis is the inflammation of the back of the throat (pharynx), commonly resulting in a sore throat. It is a frequently encountered condition that prompts individuals to seek medical advice.
Classification
Acute pharyngitis can be categorized based on its underlying cause:
4.8K
Acute Respiratory Failure-I01:21

Acute Respiratory Failure-I

Acute respiratory failure is a condition characterized by the inability of the lungs to perform their primary function: gas exchange. This failure leads to insufficient oxygen levels (hypoxemia) in the blood, elevated carbon dioxide levels (hypercapnia), or both, causing critical impairment in organ function.
Definition: It is defined by specific criteria based on blood gas measurements. Hypoxemia happens when the partial pressure of oxygen (PaO2) falls below 60 mmHg. At the same time,...
1.2K
Acute Respiratory Failure-II01:21

Acute Respiratory Failure-II

Type I Respiratory Failure, or hypoxemic respiratory failure, occurs when the partial pressure of oxygen (PaO2) in arterial blood falls below 60 mmHg while breathing room air without a corresponding increase in arterial carbon dioxide levels (PaCO2). This condition highlights a significant impairment in the lungs' capacity to oxygenate the blood.
The underlying physiological abnormalities that contribute to hypoxemic respiratory failure include:
1.2K
Acute Respiratory Failure-V01:29

Acute Respiratory Failure-V

The treatment for acute respiratory failure varies based on factors like the underlying cause, overall health, and severity. A collaborative healthcare team is essential for early detection, often through arterial blood gas analysis. Identifying the cause is the primary goal, with treatment strategies adjusted for ventilation/perfusion (V/Q) mismatch, shunting, or diffusion impairment.
Ensure that patients are monitored continuously for their response to therapy, including changes in...
541
Acute Respiratory Failure-III01:30

Acute Respiratory Failure-III

Hypercapnic respiratory failure, also known as Type 2 or ventilatory respiratory failure, is a severe condition characterized by the body's inability to effectively remove carbon dioxide (CO2) from the bloodstream. It leads to an arterial CO2 pressure (PaCO2) exceeding 45 mmHg and a blood pH above 7.35. This situation indicates that the body's ventilatory demand, or the ventilation needed to maintain normal PaCO2 levels, surpasses its supply or the maximum gas flow achievable without...
981
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:
3.4K