Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A human papilloma virus type 11 transcript encoding an E1--E4 protein.

M Nasseri, R Hirochika, T R Broker

    Virology
    |August 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    LKB1 inhibits HPV-associated cancer progression by targeting cellular metabolism.

    Oncogene·2016
    Same author

    Tuberculous aortitis with coronary ostial and left ventricular outflow obstruction: Unusual cause of sudden unexpected death.

    Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
    Same author

    Prevalence and correlates of cervico-vaginal clinical syndromes among women attending a health camp in Lalitpur district of Nepal.

    Kathmandu University medical journal (KUMJ)·2014
    Same author

    Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Transduction of the E6 and E7 genes of epidermodysplasia-verruciformis-associated human papillomaviruses alters human keratinocyte growth and differentiation in organotypic cultures.

    The Journal of investigative dermatology·2002
    Same author

    Clinical significance of P-glycoprotein immunohistochemistry and doxorubicin binding assay in patients with osteosarcoma.

    International orthopaedics·2002
    Same journal

    Regulation of HIV-2 Vpx/Vpr expression by the poly-proline motif: Enhancement of protein dimerization and translation.

    Virology·2026
    Same journal

    Distinct virus-specific regulation of RNA synthesis across genome segments by thogotovirus polymerases: insights from Oz virus and Dhori virus.

    Virology·2026
    Same journal

    Viral diversity in predatory insects used for biological control: Insights from a multi-country meta-viromic analysis.

    Virology·2026
    Same journal

    Unveiling the hidden virome of Sclerotinia sclerotiorum: New tools and discoveries in mycovirus detection.

    Virology·2026
    Same journal

    ASFV pDP238L negatively regulates type I interferon production via inhibiting the methylation of TBK1.

    Virology·2026
    Same journal

    Divergent poxvirus identified in a non-native black rat from Madagascar.

    Virology·2026
    See all related articles

    Human papillomavirus (HPV) type 11 DNA from genital warts was sequenced. Researchers identified a novel E1--E4 protein encoded by alternatively spliced and polyadenylated mRNAs, with unknown function.

    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Genital warts are commonly caused by human papillomavirus (HPV).
    • Understanding HPV gene expression is crucial for developing antiviral strategies.

    Purpose of the Study:

    • To characterize the viral DNA and mRNA of HPV type 11 associated with condyloma acuminatum.
    • To determine the nucleotide sequence of the major viral mRNA and identify encoded proteins.

    Main Methods:

    • Agarose gel electrophoresis and Southern blot analysis to study viral DNA.
    • cDNA library construction and sequencing.
    • Northern blot hybridization and R-loop electron microscopy to analyze mRNA.

    Main Results:

    • HPV type 11 DNA was present as high-copy-number monomeric circles.

    Related Experiment Videos

  • The most abundant viral mRNA (1.2 kb) was sequenced, revealing two exons.
  • Alternative polyadenylation sites and a conserved polyadenylation signal (AGUAAA) were identified.
  • A novel E1--E4 protein was encoded, with three single-base changes from the prototype sequence, two affecting E4 amino acids.

    • Conclusions:

    • The study elucidated the structure of a major HPV-11 mRNA and identified a novel E1--E4 protein.
    • The findings provide insights into HPV-11 gene expression and potential protein products.
    • The function of the identified E1--E4 protein remains to be determined.