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Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
Published on: October 10, 2017
Phosphoproteomic Analysis Identifies Signaling Pathways Regulated by Curcumin in Human Colon Cancer Cells
Tatsuhiro Sato1,2, Yutaka Higuchi3, Yoshio Shibagaki3
1Department of Biochemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan satot@aichi-cc.jp.
Background:
Curcumin, a major polyphenol of the spice turmeric, acts as a potent chemopreventive and chemotherapeutic agent in several cancer types, including colon cancer. Although various proteins have been shown to be affected by curcumin, how curcumin exerts its anticancer activity is not fully understood.
Materials And Methods:
Phosphoproteomic analyses were performed using SW480 and SW620 human colon cancer cells to identify curcumin-affected signaling pathways.
Results:
Curcumin inhibited the growth of the two cell lines in a dose-dependent manner. Thirty-nine curcumin-regulated phosphoproteins were identified, five of which are involved in cancer signaling pathways. Detailed analyses revealed that the mTORC1 and p53 signaling pathways are main targets of curcumin.
Conclusion:
Our results provide insight into the molecular mechanisms of the anticancer activities of curcumin and future molecular targets for its clinical application.
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