Related Experiment Video

Updated: Feb 23, 2026

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

12.1K

Expression and anti-inflammatory role of activin receptor-interacting protein 2 in lipopolysaccharide-activated

Qian Wu1, Yan Qi1, Na Wu1

  • 1Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, China.

Scientific Reports
|September 6, 2017
PubMed

Insights

Activin receptor-interacting protein 2 (ARIP2) is upregulated by lipopolysaccharide (LPS) in macrophages. ARIP2 overexpression inhibits inflammatory cytokine production and phagocytosis, suggesting an anti-inflammatory role in macrophage activation.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Lipopolysaccharide (LPS) activates macrophages, key immune cells.
  • The role of Activin receptor-interacting protein 2 (ARIP2) in macrophage activation is unclear.

Purpose of the Study:

  • To investigate the role of ARIP2 in macrophage activation induced by LPS.
  • To determine if ARIP2 influences inflammatory responses and phagocytic activity.

Main Methods:

  • Immunocytochemical staining to detect ARIP2 expression in RAW264.7 cells and mouse peritoneal macrophages.
  • Overexpression of ARIP2 in LPS-activated macrophages.
  • Measurement of cytokine production (IL-1β, TNFα), phagocytic activity, and CD14 expression.
  • Assessment of MyD88, TLR2, and TLR4 expression.

Main Results:

  • ARIP2 protein is present in macrophages and its expression is increased by LPS.
  • ARIP2 overexpression inhibited IL-1β and TNFα production, phagocytosis, and CD14 expression in LPS-activated macrophages.
  • ARIP2 overexpression did not affect MyD88, TLR2, or TLR4 expression.
  • In vivo ARIP2 overexpression reduced inflammatory cytokine production in LPS-stimulated mouse macrophages.

Conclusions:

  • ARIP2 plays an anti-inflammatory role in macrophages.
  • ARIP2 may exert its anti-inflammatory effects by inhibiting CD14 expression during LPS-induced activation.

More Related Videos

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation10:35

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

Published on: December 26, 2017

8.7K
A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces07:55

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

7.9K

Related Concept Videos

Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
17.4K
Inflammation01:38

Inflammation

Overview
62.7K
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
753
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.8K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
13.3K