Related Experiment Video
Updated: Feb 23, 2026
08:33
High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
Published on: February 8, 2017
42.9K
Mitochondrial Respiration and Oxygen Tension
Daniel S Shaw1, Karlia Meitha2, Michael J Considine1,2,3
1Centre for Plant Science, School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.
Methods in Molecular Biology (Clifton, N.J.)
|September 6, 2017
Summary
This study details methods for isolating plant mitochondria and measuring their respiration. These techniques precisely assess mitochondrial function and cellular oxygen metabolism using oxygen and carbon dioxide measurements.
Area of Science:
- Plant Physiology
- Mitochondrial Respiration
- Cellular Metabolism
Background:
- Understanding plant mitochondrial function is crucial for cellular oxygen metabolism.
- Precise measurements are needed to assess mitochondrial capacity.
Purpose of the Study:
- To describe routine methods for isolating intact plant mitochondria.
- To detail techniques for measuring respiration and oxygen tension in plant organs.
Main Methods:
- Isolation of intact mitochondria.
- Determination of respiratory electron transport.
- In vivo determination of oxygen tension.
- Measurement of CO2 production and O2 consumption for respiratory quotient calculation.
Main Results:
- Established routine methods for mitochondrial isolation and respiration measurement.
- Enabled precise in vivo determination of oxygen tension.
- Facilitated calculation of the respiratory quotient ([CO2]/[O2]).
Conclusions:
- The described methods provide a precise understanding of mitochondrial capacity and function.
- These techniques are valuable for studying cellular oxygen metabolism in plants.
Related Concept Videos
01:45Mitochondrial Membranes
17.5K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
17.5K
01:29Oxygen Requirements and Growth Patterns
1.6K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
1.6K
01:24Factors Affecting Respiration
10.0K
Respiration is a crucial physiological function involving exchanging oxygen (O2) and carbon dioxide (CO2) between an organism and its environment. Various factors can impact this essential process:
10.0K
01:27Oxygen Transport in the Blood
7.3K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
7.3K
01:41The Supercomplexes in the Crista Membrane
3.1K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
3.1K
01:23Physiological Control of Respiration
6.5K
Introduction
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
6.5K