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Basophil Activation Test for Allergy Diagnosis
Published on: May 31, 2021
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Using a CD45dim/CD123bright/HLA-DRneg phenotyping protocol to gate basophils in FC for airway allergy. CD123 does not
Salvatore Chirumbolo1, Geir Bjorklund, Antonio Vella
1University of Verona Department of Neurological and Movement Sciences, strada Le Grazie 9, 37134, Verona, Italy. salvatore.chirumbolo@univr.it.
Advances in Respiratory Medicine
|September 6, 2017
Summary
Accurate allergy diagnosis relies on reliable basophil activation tests (BATs). This report details a flow cytometry protocol using CD45dim/CD123bright/HLA-DRneg gating for improved basophil identification and BAT performance.
Area of Science:
- Immunology
- Allergy Diagnostics
- Flow Cytometry
Background:
- Respiratory medicine physicians require dependable tools for diagnosing allergies.
- Basophils from peripheral blood offer a reproducible model for allergy assessment.
- Basophil activation tests (BATs) are common allergy diagnostic tools available in most medical laboratories.
Purpose of the Study:
- To describe methodological aspects of a specific flow cytometry protocol for basophil identification.
- To highlight potential biases associated with the incorrect use of this basophil gating strategy.
- To contribute to the ongoing discussion on optimizing BATs for practical and analytical performance in allergy diagnosis.
Main Methods:
- Utilizing flow cytometry for basophil analysis.
- Implementing a gating strategy based on CD45dim/CD123bright/HLA-DRneg markers.
- Focusing on technical and methodological considerations for basophil identification.
Main Results:
- The technical report details the application of the CD45dim/CD123bright/HLA-DRneg gating panel.
- It identifies and discusses key biases that can arise from improper protocol execution.
- The report aims to refine the methodology for more accurate basophil detection.
Conclusions:
- Standardizing the CD45dim/CD123bright/HLA-DRneg gating protocol is crucial for reliable BATs.
- Addressing methodological biases can enhance the analytical performance of BATs.
- Further refinement of flow cytometry protocols is needed to establish BATs as a premier diagnostic tool for respiratory allergy.
