Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Growth Measurement: Direct Methods

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Periodontal status and microbial profiles across the clinical spectrum of liver cirrhosis.

Journal of oral microbiology·2026

Same author

Immunomodulators, associated or not with systemic antibiotics, to treat periodontitis: A 1-year multicenter, placebo-controlled, double-blind, randomized clinical trial.

Journal of periodontology·2026

Same author

Bone marrow B cell collapse promotes bone metastasis in breast cancer.

bioRxiv : the preprint server for biology·2026

Same author

Ketogenic diet synergistic reprogramming of both host and microbiome promotes tissue regeneration.

bioRxiv : the preprint server for biology·2026

Same author

Consensus Report of Group 3 of the 1st Global Consensus for Clinical Guidelines for the Rehabilitation of the Edentulous Maxilla: Advanced Diagnostic Imaging, Augmentation Techniques, and Management of Complications.

Clinical oral implants research·2026

Same author

Evaluation of the effects of a gel containing green tea and hyaluronic acid on clinical and Microbiological outcomes following periodontal surgery. A randomized, controlled, double-blinded, noninferiority trial.

Clinical oral investigations·2026

Same journal

Large Language Models and Retrieval-Augmented Platforms for the Diagnosis and Management of Periodontal Diseases: A Blinded Expert-Rated Comparative Study of 11 Systems.

Journal of clinical periodontology·2026

Same journal

Local Sodium Tanshinone IIA Sulfonate Attenuates Periodontal Bone Loss by Modulating Inflammatory, Immune and Osteoclastogenic Signalling.

Journal of clinical periodontology·2026

Same journal

Correction to ''Clinical Efficacy of Interventions Based on Professional Mechanical Plaque Removal in the Treatment of Dental Biofilm-Induced Gingivitis: A Systematic Review and Meta-Analysis''.

Journal of clinical periodontology·2026

Same journal

Extracellular Vesicles From Young Brain-Mediated Delivery of PRDX3 Ameliorates Alveolar Bone Loss in Periodontitis.

Journal of clinical periodontology·2026

Same journal

Cost Consequence Analysis of Non-Surgical Treatment Approaches for Patients With Periodontitis.

Journal of clinical periodontology·2026

Same journal

Management of Non-Plaque-Induced Gingival Conditions: A Systematic Review-Part 2: Inflammatory and Immune Conditions; Neoplasms; and Gingival Pigmentation.

Journal of clinical periodontology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 23, 2026

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Published on: July 24, 2018

Do different probing depths exhibit striking differences in microbial profiles?

Paula Juliana Pérez-Chaparro¹, John Anthony McCulloch^2,3, Elsa Masae Mamizuka²

¹Dental Research Division, Department of Periodontology, Guarulhos University, Guarulhos, Brazil.

Journal of Clinical Periodontology

|September 6, 2017

Summary

This summary is machine-generated.

Chronic periodontitis (ChP) subgingival microbiota differs significantly between shallow and deeper sites. New periodontal taxa, including uncultured species, were identified, advancing our understanding of ChP disease progression.

Keywords:

16S rDNA bacteria dental plaque high-throughput nucleotide sequencing periodontal disease

More Related Videos

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations

Published on: September 11, 2016

Related Experiment Videos

Last Updated: Feb 23, 2026

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Published on: July 24, 2018

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations

Soil Lysimeter Excavation for Coupled Hydrological, Geochemical, and Microbiological Investigations

Published on: September 11, 2016

Area of Science:

Microbiology
Periodontology
Genomics

Background:

Chronic periodontitis (ChP) is a complex oral infection.
Understanding the subgingival microbiota is crucial for ChP management.

Purpose of the Study:

To comprehensively characterize the subgingival microbiota in shallow, moderate, and deep periodontal sites of subjects with ChP.
To compare the microbiota composition between different probing depths and with sites from subjects with periodontal health (PH).

Main Methods:

Subgingival plaque samples were collected from subjects with ChP and PH, categorized by probing depth (≤3 mm, 4-6 mm, ≥7 mm).
High-throughput 16S rDNA sequencing was employed for microbial analysis.
Data were analyzed using mothur and R packages.

Main Results:

Analysis of 101 samples from 9 ChP and 7 PH subjects revealed 13 phyla, 118 genera, and 211 operational taxonomic units (OTUs).
Specific bacterial taxa, including Fretibacterium, Eubacterium, and Filifactor alocis, were associated with increased probing depth and ChP.
Genera such as Streptococcus and Corynebacterium were linked to periodontal health.

Conclusions:

Significant differences in subgingival microbiota were observed between shallow and moderate/deep sites in ChP subjects.
Distinct microbial profiles were also noted between shallow sites in PH and ChP subjects.
The study identified known oral bacteria and novel periodontal taxa, some previously uncultured, contributing to the understanding of ChP etiology.