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Decrease in insulin receptors during Friend erythroleukemia cell differentiation.

B H Ginsberg, T Brown, M Raizada

    Diabetes
    |September 1, 1979
    PubMed
    Summary
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    Friend erythroleukemia cells exhibit insulin receptors with typical mammalian properties. Upon differentiation induced by dimethylsulfoxide, insulin receptor concentration significantly decreases while maintaining binding characteristics.

    Area of Science:

    • Cell Biology
    • Molecular Endocrinology
    • Hematopoiesis

    Background:

    • Friend erythroleukemia cells serve as a model for studying erythroid differentiation.
    • Insulin receptors play crucial roles in cellular metabolism and growth.
    • Understanding receptor dynamics during differentiation is key to cellular regulation.

    Purpose of the Study:

    • To characterize the insulin receptor on Friend erythroleukemia cells.
    • To investigate the changes in insulin receptor concentration during induced differentiation.
    • To determine the properties of insulin receptors post-differentiation.

    Main Methods:

    • Characterization of insulin receptor binding kinetics and properties.
    • Induction of erythroid differentiation using dimethylsulfoxide.

    Related Experiment Videos

  • Quantification of insulin receptor sites per cell before and after differentiation.

    • Main Results:

    • Friend erythroleukemia cells possess insulin receptors with standard mammalian characteristics, including specific binding, temperature and pH dependence, and negative cooperativity.
    • A high number of insulin receptors (17,200 sites/cell) were initially present.
    • After dimethylsulfoxide-induced differentiation (80% benzidine-positive cells), insulin receptor concentration decreased to 4300 sites/cell.
    • The remaining receptors maintained their original binding properties.
    • The decrease in receptor number was not directly caused by dimethylsulfoxide or cell size changes.

    Conclusions:

    • Friend erythroleukemia cells express functional insulin receptors similar to those in mammals.
    • Erythroid differentiation in these cells leads to a substantial reduction in insulin receptor concentration.
    • The observed decrease in insulin receptors during differentiation is an intrinsic cellular process, not an artifact of the inducer or cell morphology.