[Effect of Kruppel-like factor 2 (KLF2) over-expression on activities of chicken PPARγ and C/EBPα promoters]

Objective To examine the effect of Kruppel-like factor 2 (KLF2) over-expression on the activities of chicken peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer binding protein alpha (C/EBPα) promoters. Methods Luciferase reporter assay was used to investigate the effect of KLF2 over-expression on reporter activities of 6 kinds of chicken PPARγ promoter construct and 5 kinds of chicken C/EBPα promoter construct in DF1 cells. Results KLF2 over-expression significantly inhibited the reporter activities of 4 kinds of chicken PPARγ promoter construct (-1978/-82, -1513/-82, -1254/-82 and -1019/-82), but had no significant effect on the reporter activities of 2 kinds of PPARγ promoter construct (-513/-82 and -320/-82). In addition, the effect of KLF2 over-expression on the reporter activities of 2 kinds of PPARγ promoter construct (-1513/-82 and -1254/-82) was significantly greater than that on the other 2 kinds of PPARγ promoter construct (-1978/-82 and -1019/-82). Additionally, KLF2 over-expression inhibited the reporter activity of one kind of C/EBPα promoter construct (-1863/+332), and enhanced the reporter activities of 4 kinds of C/EBPα promoter construct (-1318/+332, -891/+332, -538/+332 and -123/+332). There was no significant difference in the promoting effect of KLF2 over-expression among the 4 kinds of C/EBPα promoter construct (-1318/+332, -891/+332, -538/+332 and -123/+332). Conclusion The effect of KLF2 over-expression on the activities of chicken PPARγ and C/EBPα promoters are different among the constructs containing various lengths.