Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enabling Variant Proteogenomic Assay Development Through a Peptide Target Selection Pipeline and Web Application.

Proteomics·2026

Same author

Lipidomic Analysis of Plasma Extracellular Vesicles from Adiponectin Deficient Mice or Metabolic Syndrome Patients Reveals Pro-Oxidative and Pro-Inflammatory Lipid Signatures Correlating with Metabolic Dysfunction.

Journal of extracellular vesicles·2026

Same author

IRP1 deficiency alters mitochondrial metabolism and protects against metabolic syndrome pathologies.

JCI insight·2026

Same author

Plasma proteomic profiles of patients during oral anti-coagulant treatment with vitamin K antagonists.

British journal of haematology·2025

Same author

A Hit Prioritization Strategy for Compound Library Screening Using LiP-MS and Molecular Dynamics Simulations Applied to KRas G12D Inhibitors.

Analytical chemistry·2025

Same author

MRMAssayDB: a comprehensive integrated resource for targeted proteomics assays.

Expert review of proteomics·2025

Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026

Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026

Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026

Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026

Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026

Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 23, 2026

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI

Published on: August 18, 2017

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI).

Huiyan Li¹, Robert Popp¹, Bjorn Frohlich¹

¹University of Victoria-Genome BC Proteomics Centre.

Journal of Visualized Experiments : Jove

|September 6, 2017

Summary

This summary is machine-generated.

Immuno-Matrix-Assisted Laser Desorption/Ionization (iMALDI) enhances protein quantification in biofluids by using antibody-functionalized magnetic beads for analyte enrichment prior to Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry (MALDI-TOF MS). This method simplifies sample preparation and improves sensitivity for low-abundance disease biomarkers.

More Related Videos

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Related Experiment Videos

Last Updated: Feb 23, 2026

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI

Published on: August 18, 2017

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Area of Science:

Analytical Chemistry
Biomarker Discovery
Mass Spectrometry

Background:

Mass spectrometry (MS) is a key technology for protein quantification, but struggles with low-abundance analytes in complex samples like plasma.
Many disease-relevant proteins exist at low ng/mL or pg/mL concentrations, posing a challenge for direct MS detection.
Immuno-enrichment techniques improve MS sensitivity by concentrating target molecules before analysis.

Purpose of the Study:

To present a novel immuno-Matrix-Assisted Laser Desorption/Ionization (iMALDI) technology for sensitive protein and peptide quantification in biofluids.
To demonstrate the application of iMALDI for measuring low-abundance peptide angiotensin I (Ang I) in human plasma.
To evaluate the efficiency and throughput of the iMALDI assay compared to existing immuno-MS methods.

Main Methods:

Developed iMALDI assay utilizing anti-peptide antibodies functionalized on magnetic beads for immuno-enrichment.
Performed direct Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry measurement on captured analytes without prior elution.
Automated the sample preparation workflow using a liquid handling system for improved reproducibility and throughput.

Main Results:

The iMALDI assay successfully quantified the peptide angiotensin I (Ang I) in plasma samples.
The method demonstrated simplified sample preparation and rapid MALDI measurement compared to other immuno-MS assays.
Automation of the sample preparation led to enhanced assay reproducibility and higher throughput.

Conclusions:

iMALDI is an effective technology for sensitive quantification of low-abundance peptides and proteins in complex biofluid matrices.
The assay offers a simplified, automated workflow with improved reproducibility and speed for biomarker analysis.
This technique holds promise for clinical applications, such as screening for primary aldosteronism via Ang I measurement.