Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:19

Cell Migration

6.8K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Macroscale and microscale mechanical and biochemical properties of the equine superficial digital flexor tendon are regionally dependent.

American journal of veterinary research·2026
Same author

Influence of liquid repellency and slipperiness on blood-material interactions.

Journal of materials chemistry. B·2026
Same author

From saccharides to synthetics: exploring biomaterial scaffolds as cell transduction enhancers.

Biomaterials science·2025
Same author

Distinguishing the Metabolic Effect of Fetal and Adult Fibrinogen on Human Fibroblast Cell Culture by IR-MALDESI Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2025
Same author

Colloidal-fibrillar composite gels demonstrate structural reinforcement, secondary fibrillar alignment, and improved vascular healing outcomes.

Communications engineering·2025
Same author

Simple design for membrane-free microphysiological systems to model the blood-tissue barriers.

Organs-on-a-chip·2025
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Feb 23, 2026

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
06:10

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

Published on: August 16, 2017

8.2K

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments.

Seema Nandi1, Ashley C Brown2

  • 1Joint Department of Biomedical Engineering, North Carolina State University and The University of North Carolina - Chapel Hill; Comparative Medicine Institute, North Carolina State University.

Journal of Visualized Experiments : Jove
|September 6, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a novel 3D in vitro model for wound healing, improving upon 2D scratch assays. It accurately assesses cell migration in a physiologically relevant environment, aiding drug development.

More Related Videos

Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

24.3K
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

23.3K

Related Experiment Videos

Last Updated: Feb 23, 2026

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments
06:10

Characterizing Cell Migration Within Three-dimensional In Vitro Wound Environments

Published on: August 16, 2017

8.2K
Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

24.3K
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

23.3K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Wound Healing Research

Background:

  • Current in vitro wound healing models, like 2D scratch assays, lack physiological relevance.
  • Cellular behavior significantly differs between 2D and 3D environments.
  • There is a critical need for advanced 3D in vitro models to study wound closure accurately.

Purpose of the Study:

  • To develop and validate a 3D in vitro model for studying cell migration in wound healing.
  • To provide a more physiologically accurate alternative to 2D scratch assays.
  • To evaluate the impact of pro- or anti-migratory factors on cell behavior in a 3D matrix.

Main Methods:

  • A 3D model was created using spheroid bodies embedded within a fibrin matrix.
  • Cell outgrowth and migration from the spheroid were observed and quantified.
  • Brightfield microscopy and spheroid body area analysis were employed for quantification.

Main Results:

  • The 3D model successfully demonstrated and quantified cell migration (outgrowth) from spheroid bodies.
  • The system allowed for the evaluation of factors influencing cell migration.
  • This method offers a simple yet effective way to analyze cell migration in 3D matrices.

Conclusions:

  • The developed 3D model offers a more physiologically relevant approach to studying in vitro wound healing.
  • This method enhances the predictive value of in vitro studies before progressing to in vivo animal models.
  • It provides a valuable tool for researchers investigating cell migration in wound-associated matrices.