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Related Experiment Videos

Clonal analysis using recombinant DNA probes from the X-chromosome.

B Vogelstein, E R Fearon, S R Hamilton

    Cancer Research
    |September 15, 1987
    PubMed
    Summary

    Restriction fragment length polymorphisms and methylation patterns of X-chromosome genes can determine human tumor clonal composition. Specific X-chromosome probes, including those for hypoxanthine phosphoribosyltransferase and phosphoglycerate kinase, accurately reflect tumor clonality.

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    Area of Science:

    • Genetics
    • Oncology
    • Molecular Biology

    Background:

    • Restriction fragment length polymorphisms (RFLPs) and DNA methylation patterns are established tools in molecular biology.
    • X-chromosome inactivation is a key process in female mammals.
    • Determining the clonal composition of human tumors is crucial for understanding cancer development and treatment.

    Purpose of the Study:

    • To evaluate the utility of X-chromosome probes for analyzing the clonal composition of human tumors.
    • To identify specific X-chromosome genes suitable for clonal analysis in diverse populations.

    Main Methods:

    • Utilizing restriction fragment length polymorphisms (RFLPs) of X-chromosome genes.
    • Analyzing DNA methylation patterns in conjunction with RFLPs.

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  • Employing probes for the hypoxanthine phosphoribosyltransferase (HPRT) and phosphoglycerate kinase (PGK) genes.
  • Testing the method on 92 human tumors.

    • Main Results:

    • Several X-chromosome probes were found effective for clonal analysis.
    • Probes for HPRT and PGK genes were applicable in over 50% of American females.
    • Observed X-inactivation patterns accurately reflected tumor clonality in more than 95% of tested tumors.

    Conclusions:

    • X-chromosome probes, combined with methylation analysis, provide a reliable method for determining human tumor clonal composition.
    • The HPRT and PGK genes are valuable targets for this type of analysis.
    • This approach offers a high accuracy rate for assessing tumor clonality.