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Related Experiment Videos

Enhancing Upconversion Fluorescence with a Natural Bio-microlens.

Yuchao Li1, Xiaoshuai Liu1, Xianguang Yang1

  • 1Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Nanophotonics, Jinan University , Guangzhou 511443, China.

ACS Nano
|September 6, 2017
PubMed
Summary
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Living cells act as natural bio-microlenses, significantly boosting upconversion fluorescence for enhanced biomedical imaging. This breakthrough improves sensitivity for detecting pathogenic bacteria like E. coli.

Area of Science:

  • Biophotonics
  • Nanotechnology
  • Cell Biology

Background:

  • Upconversion fluorescence offers unique properties for biomedical applications but suffers from low efficiency (<1%).
  • Enhancing luminescence efficiency is crucial for practical applications in biophotonics and bioimaging.

Purpose of the Study:

  • To enhance upconversion fluorescence efficiency using living cells as bio-microlenses.
  • To achieve sensitive single-cell imaging and real-time detection of pathogenic bacteria.

Main Methods:

  • Utilized living yeast or human cells as natural bio-microlenses, stably trapped on a fiber probe.
  • Employed core-shell NaYF4:Yb3+/Tm3+ nanoparticles for upconversion fluorescence.
  • Concentrated excitation light using the bio-microlens to boost fluorescence.
Keywords:
bio-microlensbioimagingnanoparticlesoptical trappingupconversion fluorescence

Related Experiment Videos

Main Results:

  • Achieved a two-orders-of-magnitude enhancement in upconversion fluorescence intensity.
  • Successfully performed single-cell imaging and real-time detection of Escherichia coli and Staphylococcus aureus in dark fields.
  • Demonstrated a biocompatible and sensitive approach for biological detection.

Conclusions:

  • Living cells can effectively serve as bio-microlenses to significantly enhance upconversion fluorescence.
  • This method provides a powerful tool for sensitive biological imaging, biophotonic sensing, and single-cell analysis.
  • The approach is biocompatible, sensitive, and miniature, paving the way for advanced diagnostics.