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Updated: Feb 23, 2026

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
Associations between adiposity and repetitive element DNA methylation in healthy postmenopausal women
Devon J Boyne1,2, Christine M Friedenreich1,2,3, John B McIntyre4
1Department of Cancer Epidemiology & Prevention Research, Cancer Control Alberta, Alberta Health Services, Calgary, AB, Canada.
Aim:
To describe the association between adiposity and repetitive element DNA methylation in healthy postmenopausal women.
Patients & Methods:
A cross-sectional study was conducted using baseline information from 289 women who participated in the Alberta Physical Activity and Breast Cancer Prevention trial.
Results:
After adjusting for important confounders, long interspersed nuclear element-1 methylation was positively associated with intra-abdominal fat area (p = 0.03), body fat percent (p = 0.048), fat mass (p = 0.01), waist circumference (p = 0.03), hip circumference (p = 0.001), BMI (p = 0.03), current weight (p = 0.002), weight at age 20 (p = 0.02) and adulthood weight gain (p = 0.03). No significant associations were found between any of the adiposity measures and Alu methylation.
Conclusion:
Current and historical adiposity measures are positively associated with long interspersed nuclear element-1 methylation in healthy postmenopausal women.
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