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Related Experiment Video

Updated: Feb 23, 2026

Engineering Cell-permeable Protein
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Solid-phase reverse transfection for intracellular delivery of functionally active proteins.

Ruben Bulkescher1, Vytaute Starkuviene1,2, Holger Erfle1

  • 1BioQuant, Ruprecht-Karls-University Heidelberg, 69120 Heidelberg, Germany.

Genome Research
|September 7, 2017
PubMed
Summary
This summary is machine-generated.

We developed a novel solid-phase reverse transfection method for delivering functional proteins into mammalian cells. This technique simplifies intracellular protein delivery for applications like gene editing and antibody-based inhibition.

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Area of Science:

  • Cell biology
  • Molecular biology
  • Biotechnology

Background:

  • Delivering large, functional biomolecules into cells is difficult.
  • Existing methods for intracellular delivery often face challenges in preserving biomolecule function.

Purpose of the Study:

  • To develop a novel method for efficient intracellular delivery of proteins into mammalian cells.
  • To ensure the preserved function of delivered proteins for experimental applications.

Main Methods:

  • Developed a solid-phase reverse transfection technique.
  • Utilized pre-fabricated "ready to transfect" substrates carrying diverse proteins.

Main Results:

  • Successfully delivered large and functionally active biomolecules into mammalian cells.
  • Demonstrated diverse applications including antibody-mediated protein inhibition and CRISPR/Cas9 gene editing.
  • Enabled prefabrication and distribution of protein-carrying substrates.

Conclusions:

  • The novel solid-phase reverse transfection method offers an efficient way for intracellular protein delivery.
  • This technique standardizes biological assays and facilitates diverse applications in cell biology.
  • The method supports the distribution and use of pre-fabricated transfection substrates across laboratories.