Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.1K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.1K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

8.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.6K
Organization of Genes02:07

Organization of Genes

73.9K
Overview
73.9K
Operons02:09

Operons

54.7K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
54.7K
RNA Splicing01:32

RNA Splicing

60.8K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.8K
Complementary DNA01:44

Complementary DNA

31.9K
Overview
31.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disrupted energy metabolism is associated with retinal ganglion cell degeneration in autosomal dominant optic atrophy.

Science advances·2026
Same author

Correction: Utilizing TP53 hotspot mutations as effective predictors of gemcitabine treatment outcome in non-small-cell lung cancer.

Cell death discovery·2025
Same author

Utilizing TP53 hotspot mutations as effective predictors of gemcitabine treatment outcome in non-small-cell lung cancer.

Cell death discovery·2025
Same author

Comparison of Clinical Characteristics and Mortality Outcome in Critical COVID-19 Patients Infected with Alpha and Omicron Variants.

Infection and drug resistance·2025
Same author

Recommendation for the use of respiratory syncytial virus vaccines.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi·2025
Same author

Superior persistence of ustekinumab compared to anti-TNF in vedolizumab-experienced inflammatory bowel diseases patients: a real-world cohort study.

BMC gastroenterology·2024

Related Experiment Video

Updated: Feb 23, 2026

A Web Tool for Generating High Quality Machine-readable Biological Pathways
08:01

A Web Tool for Generating High Quality Machine-readable Biological Pathways

Published on: February 8, 2017

18.6K

FunctionAnnotator, a versatile and efficient web tool for non-model organism annotation.

Ting-Wen Chen1,2, Ruei-Chi Gan1,2, Yi-Kai Fang3

  • 1Bioinformatics Center, Chang Gung University, Taoyuan, Taiwan.

Scientific Reports
|September 7, 2017
PubMed
Summary

FunctionAnnotator is a new web tool that provides comprehensive functional annotations for novel transcripts. It efficiently supports research on non-model organisms and environmental samples without a reference genome.

More Related Videos

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

20.6K
Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information
09:37

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

Published on: August 15, 2019

10.5K

Related Experiment Videos

Last Updated: Feb 23, 2026

A Web Tool for Generating High Quality Machine-readable Biological Pathways
08:01

A Web Tool for Generating High Quality Machine-readable Biological Pathways

Published on: February 8, 2017

18.6K
Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
08:09

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

20.6K
Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information
09:37

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

Published on: August 15, 2019

10.5K

Area of Science:

  • Bioinformatics
  • Genomics
  • Computational Biology

Background:

  • High-throughput sequencing generates vast transcriptome data, especially for organisms lacking genomic information.
  • Identifying the functions of novel transcripts is crucial for biological studies.
  • Existing annotation tools may not adequately address the needs of non-model organisms or environmental samples.

Purpose of the Study:

  • To introduce FunctionAnnotator, a web-based tool for comprehensive transcriptome annotation.
  • To accelerate the functional annotation process using optimized computation and parallel processing.
  • To demonstrate the utility of FunctionAnnotator for non-model organisms and metatranscriptomic studies.

Main Methods:

  • FunctionAnnotator offers GO term assignment, enzyme annotation, domain/motif identification, and subcellular localization prediction.
  • The tool utilizes optimized computation and parallel computing for efficient annotation.
  • It is designed for versatility, generating outputs for various downstream analyses.

Main Results:

  • FunctionAnnotator successfully annotates transcripts from organisms without reference genomes.
  • The tool can estimate taxonomic composition in environmental samples.
  • It aids in novel protein identification when combined with proteomics data.

Conclusions:

  • FunctionAnnotator provides efficient and comprehensive transcriptome annotation.
  • It significantly benefits research on non-model organisms and metatranscriptomes.
  • This web service facilitates studies involving organisms with limited genomic resources.