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Related Experiment Video

Updated: Feb 23, 2026

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
07:28

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Viscous hydrophilic injection matrices for serial crystallography.

Gabriela Kovácsová1, Marie Luise Grünbein1, Marco Kloos1

  • 1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Jahnstrasse 29, Heidelberg 69120, Germany.

Iucrj
|September 7, 2017
PubMed
Summary

Researchers explored new viscous media for serial crystallography, a technique using X-ray free-electron lasers (XFELs). Novel hydro-gels like sodium carboxymethyl cellulose and Pluronic F-127 offer stable streams for radiation-sensitive samples.

Keywords:
XFELhigh-throughput serial crystallographyhigh-viscosity extrusionmicrocrystal injectionroom-temperature crystallography

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Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • Serial crystallography enables studying radiation-damage-prone systems using microcrystals at room temperature.
  • Efficient crystal delivery is crucial for serial crystallography at synchrotron and X-ray free-electron laser (XFEL) sources.
  • Current viscous media, like lipidic cubic phase (LCP), have limitations in chemical compatibility.

Purpose of the Study:

  • To identify and evaluate novel viscous injection media for serial crystallography.
  • To find matrices with broad chemical compatibility and reliable injection properties for sensitive protein crystals.
  • To assess the suitability of new hydro-gels for time-resolved X-ray measurements.

Main Methods:

  • Investigated sodium carboxymethyl cellulose and Pluronic F-127 as viscous injection matrices.
  • Analyzed the stability and velocity of extruded streams from these hydro-gels.
  • Measured the dependence of stream velocity on flow rate for the novel matrices.

Main Results:

  • Both sodium carboxymethyl cellulose and Pluronic F-127 demonstrated compatibility with various crystallization conditions.
  • These hydro-gels produced acceptable X-ray background.
  • The new matrices yielded very stable, adjustable streams suitable for time-resolved experiments, unlike previously characterized media.

Conclusions:

  • Sodium carboxymethyl cellulose and Pluronic F-127 are effective viscous injection media for serial crystallography.
  • These novel hydro-gels overcome limitations of existing media, offering broader compatibility and stable stream properties.
  • The findings support advancements in time-resolved structural studies of sensitive biological samples.