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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
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Visualizing old biological traces on different materials without using chemicals.

V Sterzik1, P Hinderberger2, S Panzer3

  • 1Institute of Forensic Medicine, University of Zurich, Winterthurerstrasse 190/52, 8057, Zurich, Switzerland. vera.sterzik@irm.uzh.ch.

International Journal of Legal Medicine
|September 7, 2017
PubMed
Summary
This summary is machine-generated.

Forensic investigators can detect and visualize 2-year-old biological traces like blood and semen using alternative light sources and filters. This study documents the optical behavior of aged biological samples on various materials.

Keywords:
Alternative light source (ALS)Biological stainBloodForensic light sourceForensic photographyInfrared photographyLumatec Superlite 410PerspirationSalivaSemenUrine

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Area of Science:

  • Forensic Science
  • Trace Evidence Analysis
  • Biomolecular Detection

Background:

  • Criminal investigations often require analyzing aged biological evidence, particularly in delayed discovery cases or cold cases.
  • The persistence and detectability of biological traces over time are critical factors in forensic investigations.
  • Previous research has focused on recent biological samples, leaving a gap in understanding the detectability of aged traces.

Purpose of the Study:

  • To investigate the detection and visualization of 2-year-old biological traces.
  • To document and compare the optical behavior of aged biological samples with recent ones.
  • To assess the impact of various materials on the detectability of aged biological evidence.

Main Methods:

  • Utilized an alternative light source (ALS) with different filters for visualization.
  • Collected and aged samples of blood, semen, urine, saliva, and sweat for two years.
  • Applied aged and recent biological samples to 19 distinct materials for comparative analysis.

Main Results:

  • Successfully documented the optical behavior of 2-year-old biological traces.
  • Demonstrated that aged biological samples exhibit distinct optical properties compared to recent samples.
  • Analysis revealed variations in detectability based on the type of biological material and the substrate material.

Conclusions:

  • Alternative light sources and filters are viable tools for detecting aged biological evidence.
  • The optical characteristics of biological traces change over a two-year period.
  • Understanding these changes is crucial for effective forensic analysis of old crime scene evidence.