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Related Experiment Video

Updated: Feb 23, 2026

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
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Photoacoustic thermal flowmetry with a single light source.

Wei Liu1, Bangxin Lan1, Leo Hu1

  • 1Duke University, Department of Biomedical Engineering, Durham, North Carolina, United States.

Journal of Biomedical Optics
|September 7, 2017
PubMed
Summary
This summary is machine-generated.

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This study introduces photoacoustic thermal flowmetry using optical-resolution photoacoustic microscopy (OR-PAM) to measure flow speed. The method accurately quantifies blood flow in microvasculature by analyzing photoacoustic signal dynamics.

Area of Science:

  • Biomedical Optics
  • Photoacoustics
  • Microfluidics

Background:

  • Accurate measurement of fluid flow, especially in microvasculature, is crucial for physiological and pathological studies.
  • Existing flowmetry techniques may have limitations in resolution, invasiveness, or applicability to micro-scale environments.

Purpose of the Study:

  • To develop and validate a novel photoacoustic thermal flowmetry technique for precise flow speed measurement.
  • To establish a mathematical model correlating photoacoustic signal dynamics with fluid flow speed.
  • To demonstrate the method's efficacy in both in vitro and in vivo settings.

Main Methods:

  • Utilized optical-resolution photoacoustic microscopy (OR-PAM) with a single laser source for simultaneous thermal tagging and photoacoustic excitation.
Keywords:
blood flowoptical-resolution photoacoustic microscopyphotoacoustic imagingthermal flowmetrythermal tagging

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  • Developed a lumped mathematical model to describe the relationship between photoacoustic signal rise time and flow speed.
  • Performed proof-of-concept experiments with flowing blood in a tube and validated in vivo in mouse ear microvasculature.

    • Main Results:

    • Demonstrated a linear relationship between the rising time constant of photoacoustic signals and the medium's flow speed.
    • Achieved high accuracy (approximately ±6%) in flow speed measurements.
    • Successfully quantified blood flow speeds in the microvasculature of a mouse ear.

    Conclusions:

    • Photoacoustic thermal flowmetry based on OR-PAM is a viable, accurate, and non-invasive method for measuring flow speed.
    • The developed mathematical model enables precise quantification of flow dynamics.
    • This technique holds significant potential for biomedical research and clinical diagnostics involving microcirculation.